| Name | Nimbolide |
| Description | Nimbolide, a triterpenoid derived from neem leaves and flowers, is a natural product and a CDK4/CDK6/NF-κB inhibitor that inhibits multiple signaling pathways including Wnt and PI3K-Akt, possesses antitumor activity, and can induce tumor cell apoptosis. |
| In vitro | Methods:BV-2 mouse microglial cells were pretreated with Nimbolide (125, 250, 500 nM) for 30 minutes, then co-incubated with LPS (100 ng/mL) for 24 hours. Detection was performed using ELISA, Griess assay, and Western blot.
Results: Nimbolide concentration-dependently reduced levels of TNFα, IL-6, IFNγ, NO/iNOS, and PGE2/COX-2, inhibited NF-κB and MAPK pathways, and activated the Nrf2 antioxidant mechanism.[1]
Methods: Nine H. pylori strains including G27, 26695, and J99 were incubated with Nimbolide at 0.625–40 μg/mL for 24 hours. In vitro activity was evaluated through MIC/MBC determination and bactericidal kinetics assays.
Results: Nimbolide exhibited MIC values of 1.25–2.5 μg/mL and MBC values of 2.5–10 μg/mL against tested strains, demonstrating time- and concentration-dependent bactericidal effects. It showed synergistic activity at low pH and was effective against multidrug-resistant strains.[2]
Methods: Human bladder cancer EJ and 5637 cells were incubated with Nimbolide at 0, 0.5, 1, and 3 μmol/L for 12 hours. Detection was performed using MTT assay, cell counting, flow cytometry, wound healing assay, Transwell invasion assay, gelatin zymography, and EMSA.
Results: Nimbolide concentration-dependently inhibited cell viability (IC?? approximately 3 μmol/L), induced G2/M phase arrest, promoted JNK phosphorylation, inhibited p38MAPK and AKT phosphorylation, and reduced MMP-9 activity as well as NF-κB, AP-1, and Sp-1 transcription factor binding activity.[3] |
| In vivo | Methods: A Freund's complete adjuvant-induced arthritis model was established in male Wistar rats. Nimbolide was administered orally at a dose of 20 mg/kg/day with DMSO as the vehicle control, continuously until day 25.
Results: Nimbolide significantly reduced joint scores, paw swelling, and organ indices, alleviated histopathological damage, and downregulated expression of inflammatory factors including TNF-α and IL-6 as well as iNOS and NF-κB proteins.[4]
Methods: DSS-induced acute colitis and IL-10?/? chronic colitis mouse models were employed. Nimbolide was administered by gavage at doses of 0.2 and 1 mg/kg/day with DMSO as the vehicle, continuously for 7 or 14 days.
Results: Nimbolide significantly improved weight loss, colon shortening, disease activity index, and histological scores, and inhibited IκBα phosphorylation in colon tissue.[5] |
| Storage | Keep away from direct sunlight,Keep away from moisture,Store at low temperature,Store under nitrogen,
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 60 mg/mL (128.61 mM), Sonication and heating are recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 1 mg/mL (2.14 mM), Sonication is recommended.
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| Keywords | Nuclear factor-κB | Nuclear factor-kappaB | Nimbolide | NF-κB | NFκB | NF-kB | NFkB | Inhibitor | inhibit | Cyclin dependent kinase | CDK6 | CDK4 | CDK | Apoptosis |
| Inhibitors Related | Stavudine | Aceglutamide | Urea | Tamoxifen | Cysteamine hydrochloride | Metronidazole | Ethyl linoleate | D-Psicose | Formamide | Dimethyl phthalate | Alginic acid | Sildenafil citrate |
| Related Compound Libraries | Anti-Tumor Natural Product Library | Terpene Natural Product Library | Bioactive Compound Library | Traditional Chinese Medicine Monomer Library | Kinase Inhibitor Library | Selected Plant-Sourced Compound Library | Natural Product Library | Inhibitor Library | Natural Product Library for HTS | Anti-Aging Compound Library | Immunology/Inflammation Compound Library | Bioactive Compounds Library Max |
United States