Product Details
| Product Name:
SPQ |
CAS No.:
83907-40-8 |
| Purity:
99.51% |
Supply Ability:
10g |
| Release date:
2026/05/11 |
Product Introduction
Bioactivity
| Name | SPQ |
| Description | SPQ is used to measure membrane chloride transport mechanisms. |
| Cell Research | Instructions
a. Solution preparation:
1. Preparation of SPQ stock solution: Dissolve SPQ powder in water or buffer to prepare a stock solution of at least 1 mg/mL. For example, weigh 10 mg of SPQ and add it to 10 mL of water or buffer.
2. Preparation of working solution: Dilute SPQ stock solution with Krebs-HEPES buffer (20 mM HEPES, 128 mM NaCl, 2.5 mM KCl, 2.7 mM CaCl2, 1 mM MgCl2, 16 mM glucose, pH 7.4) to prepare a 5-10 μM working solution for later use.
Note: The stock solution needs to be stored at -20℃ or -80℃. Please avoid repeated freezing and thawing. The diluted working solution should be prepared and used as soon as possible.
b. Operation steps:
1. Cultivate the cells to be tested to an appropriate density, about 8-10×10^6 cells/mL.
2. Wash the cells 3 times with Krebs-HEPES buffer.
3. Add 5-10 μM SPQ in Krebs-HEPES buffer to the cells and incubate at 37°C for 1 hour.
4. Wash the cells 5 times with Krebs-HEPES buffer to remove the residual dye outside the cells.
5. Observe the cells using a fluorescence microscope with an excitation wavelength of 344 nm and an emission wavelength of 443 nm.
The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| Storage | Keep away from direct sunlight
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | H2O : 20 mg/mL (71.09 mM), Sonication is recommended.
|
| Keywords | SPQ | Inhibitor | inhibit |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
Recommended supplier
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Update time |
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$99.00
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Amikon Limited
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2019-09-06 |
United States