Tri (2-carboxyethyl) phosphine hydrochloride (TCEP hydrochloride) is a new reductant and deprotector of thiolated DNA. Sulfur atoms at the end of thiolated DNA tend to form dimers in solution, especially in the presence of oxygen. This dimerization greatly reduces the efficiency of some coupling reaction experiments (such as DNA fixation in biosensors); The dimerization of DNA can be reduced by adding tri (2-carboxyethyl) phosphine hydrochloride (TCEP hydrochloride) to the DNA solution and removing it after a period of reaction.

Tri (2-carbonylethyl) phosphate hydrochloride is an organic phosphorus compound, which can be used as a water-soluble reagent.It is used for selective reduction of disulfide. It is more stable than DTT in mass spectrometry application.After alkalization, the water-soluble ligand can be simply removed from the ruthenium based metathesis catalyst by washing the crude product of the reaction in water.