Thiazole Orange NEW
| Price | $35 |
| Package | 50mg |
| Supply Ability: | 10g |
| Update Time: | 2026-05-26 |
Product Details
| Product Name: Thiazole Orange | CAS No.: 107091-89-4 |
| Purity: 99.93% | Supply Ability: 10g |
| Release date: 2026/05/26 |
Product Introduction
Bioactivity
| Name | Thiazole Orange |
| Description | Thiazole Orange is a dye that can be used for reticulocyte analysis. |
| Cell Research | Instructions I. Solution preparation 1. Stock solution: Dissolve Thiazole Orange in DMSO or ethanol to prepare a stock solution with a concentration of 13 mg/ml. Since the dye is light-sensitive, it needs to be handled and stored in the dark. 2. Working solution: Dilute the stock solution to the working concentration according to the experimental requirements, usually 1 mg/ml. Use an appropriate buffer (such as PBS) for dilution. II. Operation steps 1. Coupling with oligonucleotides: (1) Thiazole Orange can be covalently coupled to modified oligonucleotides or linkers through amino-reactive functional groups (such as NHS esters or isothiocyanates). (2) After coupling, purify the labeled oligonucleotides by high-performance liquid chromatography (HPLC) or gel electrophoresis. (3) Verify the coupling efficiency by absorption and fluorescence measurements. 2. Reticulocyte analysis: (1) Prepare a red blood cell suspension (prepared in PBS or isotonic buffer). (2) Add Thiazole Orange at a working concentration (usually 1 mg/ml) and incubate at 37°C for 60 minutes to allow it to bind to RNA in reticulocytes. (3) Analyze the stained cells using a flow cytometer or fluorescence microscope, selecting appropriate excitation wavelengths (~510 nm) and emission wavelengths (~530 nm). 3. Application of fluorescent hybridization probes: (1) Use labeled oligonucleotide probes to detect specific nucleic acid sequences in hybridization experiments. (2) Optimize hybridization conditions (such as temperature and buffer composition) to ensure specific binding of the probe to the target sequence. (3) Measure the fluorescent signal using a fluorometer or fluorescence imaging system. Notes: 1. Photosensitivity: Thiazole Orange is light-sensitive and should be avoided from prolonged exposure to strong light when used. 2. Storage conditions: The stock solution should be stored in a sealed container at -20°C to reduce moisture exposure and prevent degradation. Avoid repeated freezing and thawing. 3. Compatibility: Thiazole Orange binds strongly to nucleic acids. Avoid using solutions containing detergents or high salt concentrations, which may reduce its interaction with nucleic acids. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| Storage | Keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | H2O : < 0.1 mg/mL (insoluble) DMSO : 12.25 mg/mL (25.7 mM), Sonication is recommended. 10% DMSO+40% PEG300+5% Tween-80+45% Saline : 1 mg/mL (2.1 mM), Sonication is recommended. |
| Keywords | Thiazole Orange | reticulocytes | oligonucleotides | nucleic | Inhibitor | inhibit | dyes | conjugated | acid |
| Related Compound Libraries | Bioactive Compound Library | Bioactive Compounds Library Max |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
You may like
Recommended supplier
| Product name | Price | Suppliers | Update time | |
|---|---|---|---|---|
| $13.00 |
VIP6Y
|
Baoji Guokang Healthchem Co., Ltd.
|
2026-05-15 | |
|
VIP5Y
|
Changsha Staherb Natural Ingredients Co., Ltd.
|
2026-07-03 | ||
|
Anhuyan (Hainan) Biotechnology Co., LTD
|
2026-05-22 | |||
| $1.10 |
VIP6Y
|
Dideu Industries Group Limited
|
2025-11-18 |
United States- Since: 2011-01-07
- Address: 36 Washington Street, Wellesley Hill, MA
INQUIRY