Product Information 

Product Name:	Tris(hydroxymethyl)aminomethane
Synonyms:	OmniPur TRIS -CAS 77-86-1 - Calbiochem;Tris Base, Molecular Biology Grade - CAS 77-86-1 - Calbiochem;Tris Base, ULTROL Grade - CAS 77-86-1 - Calbiochem;Tris Buffer, 1.0 M, pH 8.0, Molecular Biology Grade - CAS 77-86-1 - Calbiochem;Tris Buffer, 100 mM, pH 7.4, Molecular Biology Grade - CAS 77-86-1 - Calbiochem;Tris(Hydroxymethyl)aminomethane (Trometamol) high purity;1-[ethyl(hydroxymethyl)amino]ethane-1,2-diol;TRIS,Tromethamine, Trometamol
CAS:	77-86-1
MF:	C4H11NO3
MW:	121.14
EINECS:	201-064-4
Product Categories:	tris;Pharmaceutical intermediates;ACS Grade Buffers;Amino Alcohols;Biological Buffers;API;Biochemistry;Reagents for Electrophoresis;Buffer;Buffers A to Z;Building Blocks;Chemical Synthesis;Organic Building Blocks;Oxygen Compounds;bc0001;77-86-1
Mol File:	77-86-1.mol

Usage 

1. Chemical raw material: Tris is widely used as an important chemical raw material for flame retardant materials, coatings and amino resins, etc.
2. Reaction medium: As an excellent reaction medium, Tris is often used in organic synthesis reactions, which can play the role of catalyst.
3. Resin cross-linking agent: Tris(hydroxymethyl)aminomethane can also be used in resin cross-linking reaction, which can improve the hardness and heat resistance of resin.
4. Biological buffering agent: In the field of medicine, Tris is used as a biological buffering agent, which is widely used in cell culture, DNA/RNA extraction, PCR amplification, etc.

Configuration method of Tris solution:
1. Weigh the required amount of Tris powder and add it to sterile high purity water.
2. Use a magnetic stirrer to fully dissolve the Tris powder until it is completely homogeneous.
3. Calculation of concentration: Generally, the concentration of trimethylolaminomethane is 1 mol/L, and the concentration can be adjusted appropriately according to the need.
4. Measure the PH value of the solution with a PH meter, which should generally be controlled between 7.0-8.5.
5. Preparation of buffer solution: standardize the solution to the required volume, usually used as a buffer solution with a concentration of 50-200 mmol/L.
6. Sterilization: The trimethylolaminomethane solution is loaded into sterile bottles and sterilized according to a certain process.

It should be noted that during the configuration of trimethylolaminomethane, chemical safety operation procedures should be strictly observed, and personal protective equipment should be worn to avoid splashing the chemical on the skin and eyes, and to avoid inhalation and accidental ingestion of the chemical. At the same time, the label and production date of the chemical should be checked before use to avoid using expired or poor quality chemicals.

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