[CAS NO. 132-92-3]??Methicillin sodium salt
PRODUCTS SPECIFICATIONS [132-92-3]
DESCRIPTION [132-92-3]
Overview
| MDL | MFCD07787409 |
|---|---|
| Molecular Weight | 402.40 |
| Molecular Formula | C17H19N2NaO6S |
| SMILES | O=C([C@H](N1C2=O)C(C)(S[C@@]1([C@@H]2NC(C3=C(C=CC=C3OC)OC)=O)[H])C)O[Na] |
4 Publications Citing Use of MCE
Summary
Methicillin sodium salt (Meticillin sodium) is a β-lactam , semi-synthetic antibiotic related to penicillin antibiotic . Methicillin sodium salt inhibits penicillin-binding proteins involved in the synthesis of peptidoglycan. Methicillin sodium salt inhibits S. aureus with a MIC value of 2.1 μg/mL. Methicillin sodium salt can be used for the research of inflammation [1] [2] .
IC50 & Target
|
β-lactam |
In Vitro
Methicillin sodium salt (100 μg/mL; 18 h) kills S. aureus after incubation intracellular for 18 hours [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [1]
| Cell Line: | Leukocytes |
| Concentration: | 100 μg/mL |
| Incubation Time: | 18 hours |
| Result: | Effectively eliminated S. aureus with minimal bactericidal concentration of 3.13 μg/mL, and exhibited better effects under aerobical condition. |
In Vivo
Methicillin sodium salt (42.5 and 85 mg/kg; i.m. four times daily; for 21 days or till spontaneous death) against the enterococcus in leukocytes
[1]
.
Methicillin sodium salt (400 mg/kg; i.p. once) survives infected mice better than compared group
[2]
.
Methicillin sodium salt (400 mg/kg; i.h. once) prevents infected mice from death
[2]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | White New Zealand rabbits, weighing 2.0 to 3.0 kg [1] |
| Dosage: | 42.5 and 85 mg/kg |
| Administration: | Intramuscular injection; 42.5 and 85 mg/kg four times daily; for 21 days or till spontaneous death |
| Result: | Inhibited enterococcus at 24 hours with a half-live of 1.1 h, but showed no statistical significance to rabbits. |
| Animal Model: | Mice with 5-7 X 10 8 S. aureus [2] |
| Dosage: | 400 mg/kg |
| Administration: | Intraperitoneal injection; 400 mg/kg; once |
| Result: | Showed a survival rate of 30.5%, higher than compared group with a survival rate of 6.9%. |
| Animal Model: | Mice with 5-7 X 10 8 S. aureus [2] |
| Dosage: | 400 mg/kg |
| Administration: | Subcutaneous injection; 400 mg/kg; once |
| Result: | Suppressed large abscesses developed in mice, and also prevented mice from death. |
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date | Phase |
|---|---|---|---|---|
| NCT03096405 | Groupe Hospitalier Paris Saint Joseph |
MRSA Bacteremia
|
February 1, 2014 |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 125 mg/mL ( 310.64 mM ; Need ultrasonic)
H 2 O : 100 mg/mL ( 248.51 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.4851 mL | 12.4254 mL | 24.8509 mL |
| 5 mM | 0.4970 mL | 2.4851 mL | 4.9702 mL |
| 10 mM | 0.2485 mL | 1.2425 mL | 2.4851 mL |
-
1.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (248.51 mM); Clear solution; Need ultrasonic
-
2.
-
3.
Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (5.17 mM); Clear solution
-
4.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.08 mg/mL (5.17 mM); Clear solution
References
- [1] Lincoln LJ, et al. Penicillinase-resistant penicillins plus gentamicin in experimental enterococcal endocarditis. Antimicrob Agents Chemother. 1977 Oct;12(4):484-9.
- [2] Mandell GL, Vest TK. Killing of intraleukocytic Staphylococcus aureus by rifampin: in-vitro and in-vivo studies. J Infect Dis. 1972 May;125(5):486-90.
Synonyms