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[CAS NO. 23491-52-3]??Hoechst33342

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PRODUCTS SPECIFICATIONS [23491-52-3]

Catalog
HY-15559
Brand
MCE
CAS
23491-52-3

DESCRIPTION [23491-52-3]

Overview

MDLMFCD00166996
Molecular Weight452.55
Molecular FormulaC27H28N6O
SMILESCN1CCN(C2=CC=C3C(N=C(C4=CC=C5C(N=C(C6=CC=C(OCC)C=C6)N5)=C4)N3)=C2)CC1

For research use only. We do not sell to patients.


Summary

Hoechst 33342 is a DNA minor groove binder used fluorochrome for visualizing cellular DNA .


IC50 & Target

Dye reagent [1]
DNA Stain [1]


In Vitro

Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove. On binding to DNA, the fluorescence greatly increases. This protocol describes the use of Hoechst 33342 to label nuclear DNA of cells grown in culture. Hoechst 33342 can also be used to stain fixed cells by substituting Hoechst 33342 for DAPI [1] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.


Appearance

Solid


Shipping

Room temperature in continental US; may vary elsewhere.


Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)


Solvent & Solubility

In Vitro:

DMSO : 6.25 mg/mL ( 13.81 mM ; ultrasonic and warming and heat to 60°C)

H 2 O : 1 mg/mL ( 2.21 mM ; ultrasonic and warming and heat to 80°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2097 mL 11.0485 mL 22.0970 mL
5 mM 0.4419 mL 2.2097 mL 4.4194 mL
10 mM 0.2210 mL 1.1049 mL 2.2097 mL
* Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one: PBS

    Solubility: 5 mg/mL (11.05 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

  • 2.

    Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

    Solubility: ≥ 0.5 mg/mL (1.10 mM); Clear solution

  • 3.

    Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 0.5 mg/mL (1.10 mM); Clear solution

  • 4.

    Add each solvent one by one: 10% DMSO >> 90% corn oil

    Solubility: ≥ 0.5 mg/mL (1.10 mM); Clear solution

* All of the co-solvents are available by MCE.


Synonyms

2,6′-Bi-1H-benzimidazole, 2′-(4-ethoxyphenyl)-6-(4-methyl-1-piperazinyl)-
2,5′-Bibenzimidazole, 2′-(p-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-
2,5′-Bi-1H-benzimidazole, 2′-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-
2′-(4-Ethoxyphenyl)-6-(4-methyl-1-piperazinyl)-2,6′-bi-1H-benzimidazole
2-[2-(4-Ethoxyphenyl)-6-benzimidazolyl]-6-(1-methyl-4-piperazinyl)benzimidazole
HOE 33342
Hoechst 33342
Bisbenzimide
Ho 342
NSC 334072
BXI 72
Hoechst H 33342
2′-(4-Ethoxyphenyl)-6-(4-methylpiperazin-1-yl)-1H,3′H-2,5′-bibenzimidazole
2-(4-Ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazole

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