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[CAS NO. 516-72-3]??20(S)-Hydroxycholesterol

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PRODUCTS SPECIFICATIONS [516-72-3]

Catalog
HY-12316
Brand
MCE
CAS
516-72-3

DESCRIPTION [516-72-3]

Overview

MDLMFCD16661190
Molecular Weight402.65
Molecular FormulaC27H46O2
SMILESC[C@@]1([C@@]2([H])[C@@](C)(O)CCCC(C)C)[C@](CC2)([H])[C@@](CC=C3[C@@]4(CC[C@H](O)C3)C)([H])[C@]4([H])CC1

For research use only. We do not sell to patients.

Summary

20(S)-hydroxyCholesterol (20α-Hydroxycholesterol) is an allosteric activator of the oncoprotein smoothened (Smo) that activates the hedgehog (Hh) signaling pathway with an EC 50 of 3 μM in a gene transcription reporter assay using NIH3T3 cells [1] [2] .


IC50 & Target

Human Endogenous Metabolite


Appearance

Solid


Shipping

Room temperature in continental US; may vary elsewhere.


Storage

Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month

Solvent & Solubility

In Vitro:

DMSO : 30 mg/mL ( 74.51 mM ; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4835 mL 12.4177 mL 24.8355 mL
5 mM 0.4967 mL 2.4835 mL 4.9671 mL
10 mM 0.2484 mL 1.2418 mL 2.4835 mL
* Please refer to the solubility information to select the appropriate solvent.


Synonyms

Cholest-5-ene-3,20-diol, (3β)-
Cholest-5-ene-3β,20-diol, (20S)-
(3β)-Cholest-5-ene-3,20-diol
20α-Hydroxycholesterol
(20S)-Hydroxycholesterol
(20S)-20-Hydroxycholesterol
Cholest-5-ene-3β,20α-diol
20S-Cholest-5-ene-3β,20-diol

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