Hydrocortisone (Cortisol)

別名: NSC 10483 中文名稱：氫化可的松（皮質醇）

目錄號：S1696 Purity: 99.99%

Hydrocortisone是一種由腎上腺產生的類固醇激素或糖皮質激素。

Hydrocortisone (Cortisol) Chemical Structure

CAS: 50-23-7

規(guī)格	價格	庫存
10mM (1mL in DMSO)	591	現貨
50mg	441	現貨
200mg	810	現貨
1g	1990	現貨
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產品質控

批次：純度： 99.99%

99.99

Hydrocortisone (Cortisol)相關產品

相關產品	Corticosterone AL082D06	點擊展開
相關化合物庫	FDA藥物庫天然產物庫已知活性藥物庫-I 外泌體分泌相關化合物庫人類激素相關化合物庫	點擊展開

細胞實驗數據示例

細胞系	實驗類型	給藥濃度	孵育時間	活性描述	文獻信息(PMID)
RAW264.7	Antiinflammatory assay	0.5 to 50 ug/ml	4 hrs	Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as inhibition of IL-10 production at 0.5 to 50 ug/ml pretreated for 4 hrs followed by challenge with 1 ug/mL LPS for 48 hrs by ELISA relative to control	21384845
RAW264.7	Antiinflammatory assay	0.5 to 50 ug/ml	4 hrs	Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as inhibition of IL-6 production at 0.5 to 50 ug/ml pretreated for 4 hrs followed by challenge with 1 ug/mL LPS for 48 hrs by ELISA relative to control	21384845
RAW264.7	Antiinflammatory assay	0.5 to 50 ug/ml	4 hrs	Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as inhibition of IL1-beta production at 0.5 to 50 ug/ml pretreated for 4 hrs followed by challenge with 1 ug/mL LPS for 48 hrs by ELISA relative to control	21384845
RAW264.7	Antiinflammatory assay	0.5 to 50 ug/ml	4 hrs	Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as inhibition of TNFalpha production at 0.5 to 50 ug/ml pretreated for 4 hrs followed by challenge with 1 ug/mL LPS for 48 hrs by ELISA relative to control	21384845
RAW264.7	Antiinflammatory assay	0.5 to 50 ug/ml	4 hrs	Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as inhibition of nitric oxide production at 0.5 to 50 ug/ml pretreated for 4 hrs followed by challenge with 1 ug/mL LPS for 48 hrs by Griess reaction method relative to control	21384845
RAW264.7	Antiinflammatory assay	10 uM	2 hrs	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced increase in TNF-alpha level at 10 uM incubated for 2 hrs prior to LPS challenge measured after 22 hrs by ELISA relative to control	25515561
RAW264.7	Antiinflammatory assay	10 uM	2 hrs	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced increase in IL-6 level at 10 uM incubated for 2 hrs prior to LPS challenge measured after 22 hrs by ELISA relative to control	25515561
RAW264.7	Antiinflammatory assay	10 uM	2 hrs	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced increase in IL-1beta level at 10 uM incubated for 2 hrs prior to LPS challenge measured after 22 hrs by ELISA relative to control	25515561
RAW264.7	Antiinflammatory assay	10 uM	2 hrs	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production at 10 uM incubated for 2 hrs prior to LPS challenge measured after 22 hrs relative to vehicle-treated control	25515561
B16F10	Apoptosis assay	20 uM	36 hrs	Induction of apoptosis in mouse B16F10 cells assessed as increase in cleaved caspase 3 level at 20 uM after 36 hrs by western blot analysis	26695732
B16F10	Apoptosis assay	20 uM	36 hrs	Induction of apoptosis in mouse B16F10 cells assessed as increase in p53 expression at 20 uM after 36 hrs by propidium iodide staining based FACS analysis	26695732
B16F10	Apoptosis assay	20 uM	36 hrs	Induction of apoptosis in mouse B16F10 cells assessed as increased Bax expression at 20 uM after 36 hrs by western blot analysis	26695732
B16F10	Apoptosis assay	20 uM	36 hrs	Induction of apoptosis in mouse B16F10 cells assessed as increase in cytochrome C level at 20 uM after 36 hrs by western blot analysis	26695732
B16F10	Antitumor activity assay	6.11 mg/kg		Antitumor activity against mouse B16F10 cells xenografted in C57BL/J mouse assessed as inhibition of tumor growth at 6.11 mg/kg, ip	26695732
mouse L929 cells	Growth inhibition assay		6 days	Growth inhibition of mouse L929 cells after 6 days	926113
mouse RAW264.7 cells	Function assay		24 h	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of IFN-gamma induced NO production after 24 hrs by Griess reaction, IC50=0.061 μM	21361338
RAW264.7	Function assay		24 hrs	Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells after 24 hrs by Griess reaction based spectrophotometry, IC50 = 40.64 μM.	21807513
RAW264.7	Function assay		24 hrs	Inhibition of LPS-induced NO production in mouse RAW264.7 cells after 24 hrs by Griess reaction, IC50 = 40.64 μM.	23067550
RAW264.7	Antiinflammatory assay		24 hrs	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay, IC50 = 43.8 μM.	23755850
L929	Function assay		20 hrs	Displacement of 1 x 10'-8 M of [1,2,3-3H]-triamcinolone acetonide from glucocorticoid receptor in soluble fraction of mouse L929 cells after 20 hrs, Kd = 0.043 μM.	926113
RAW264.7	Antiinflammatory assay			Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production by MTT assay, IC50 = 40.64 μM.	20879743
HEK293	Function assay			Inhibition of MR-mediated transactivation of galactosidase reporter gene in HEK293 cells expressing 11betaHSD1	16759088
neural precursor cells	Function assay			Inhibition of neurosphere proliferation of mouse neural precursor cells by MTT assay	17417631
點擊查看更多細胞系數據

生物活性

產品描述

Hydrocortisone是一種由腎上腺產生的類固醇激素或糖皮質激素。

靶點

Glucocorticoid receptor

體外研究(In Vitro)
體外研究活性	在人腦微血管內皮細胞系hCMEC/ D3中，Hydrocortisone防止促炎刺激（TNFα的給藥）引起的內皮屏障破壞，這可以證明是部分地維護occludin的水平。 Hydrocortisone處理的樹突細胞（DC）表現出降低的表達MHC II類分子，共刺激分子CD86和直流特異性標記CD83，以及一個大大減少的IL-12的分泌。Hydrocortisone處理的DC抑制產生IFN-γ而誘導IL-4的增加的釋放，IL-5的沒有變化。Hydrocortisone減少在樹突狀細胞的T細胞增殖。 Hydrocortisone阻止TNF-α誘導糖萼的嚴重退化，增加冠脈阻力，提高血管滲漏和通透性，羥乙基淀粉并引起離體豚鼠心臟肥大細胞脫顆粒。在離體豚鼠心臟，Hydrocortisone降低缺血后的氧化應激，灌注壓和漏出液形成。Hydrocortisone抑制多配體聚糖-1，硫酸乙酰肝素和乙酰透明質酸的缺血后脫落，以及從駐留肥大細胞組胺釋放。 Hydrocortisone增加IL-4誘導的胚系C epsilon轉錄水平增加兩倍，并提供成熟Ç小量的mRNA轉錄所需的信號。在IL-4處理的B細胞中，Hydrocortisone誘導S mu-S epsilon缺失切換重組中，并支持序貫同型從IgM經由IgG4轉換至IgE的模型。
實驗圖片	檢測方法	檢測指標	實驗圖片	PMID
	Western blot	parkin / AIMP2 CREB		28366931
	Immunofluorescence	Glut2		29717618
	Growth inhibition assay	Cell viability		22829975

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
臨床試驗信息 (data from https://clinicaltrials.gov, updated on 2024-05-22)
NCT05741762	Active not recruiting	Critical Illness\|Adrenal Insufficiency\|Septic Shock	Dr Adnan Agha\|United Arab Emirates University	January 31 2023	--
NCT05607901	Recruiting	Dermatologic Disease	Tanta University	October 28 2022	Phase 2
NCT05324618	Completed	Atopic\|Dermatitis	Ain Shams University\|National Hepatology & Tropical Medicine Research Institute	May 15 2022	Phase 4

參考文獻
[1]https://pubmed.ncbi.nlm.nih.gov/18258663/ [2]https://pubmed.ncbi.nlm.nih.gov/11496241/ [3]https://pubmed.ncbi.nlm.nih.gov/18836678/ [4]https://pubmed.ncbi.nlm.nih.gov/18073553/ [5]https://pubmed.ncbi.nlm.nih.gov/8409416/ + 展開

參考文獻

[1]https://pubmed.ncbi.nlm.nih.gov/18258663/
[2]https://pubmed.ncbi.nlm.nih.gov/11496241/
[3]https://pubmed.ncbi.nlm.nih.gov/18836678/

[4]https://pubmed.ncbi.nlm.nih.gov/18073553/
[5]https://pubmed.ncbi.nlm.nih.gov/8409416/

+ 展開

化學信息&溶解度

分子量	362.46	分子式	C₂₁H₃₀O₅
CAS號	50-23-7	SDF	Download Hydrocortisone (Cortisol) SDF
Smiles	CC12CCC(=O)C=C1CCC3C2C(CC4(C3CCC4(C(=O)CO)O)C)O
儲存條件(自收到貨起)	3年-20°C粉狀	儲備液保存和期限建議分裝儲備液，避免反復凍融！	1年-80°C溶于溶劑
儲存條件(自收到貨起)	3年-20°C粉狀	儲備液保存和期限建議分裝儲備液，避免反復凍融！	1個月-20°C溶于溶劑

體外溶解度
批次：

DMSO : 72 mg/mL ( (198.64 mM) ；DMSO吸濕會降低化合物溶解度，請使用新開封DMSO)

Ethanol : 15 mg/mL (41.38 mM)

Water : Insoluble

DMSO : 72 mg/mL ( (198.64 mM) ；DMSO吸濕會降低化合物溶解度，請使用新開封DMSO)

Ethanol : 12 mg/mL (33.1 mM)

Water : Insoluble

DMSO : 72 mg/mL ( (198.64 mM) ；DMSO吸濕會降低化合物溶解度，請使用新開封DMSO)

Ethanol : 9 mg/mL (24.83 mM)

Water : Insoluble

摩爾濃度計算器

體內溶解配方批次：現配現用，請按從左到右的順序依次添加，澄清后再加入下一溶劑				動物體內配方計算器
均勻懸濁液	CMC-NA	≥5mg/ml	以 1 mL 工作液為例，取 5 mg該產品加到 1 ml CMC-NA 溶液中，混合均勻，即可得工作液濃度為 5 mg/ml的均勻懸濁液。
均勻懸濁液	CMC-NA	≥5mg/ml	以 1 mL 工作液為例，取 5 mg該產品加到 1 ml CMC-NA 溶液中，混合均勻，即可得工作液濃度為 5 mg/ml的均勻懸濁液。
均勻懸濁液	CMC-NA	≥5mg/ml	以 1 mL 工作液為例，取 5 mg該產品加到 1 ml CMC-NA 溶液中，混合均勻，即可得工作液濃度為 5 mg/ml的均勻懸濁液。

實驗計算

摩爾濃度計算器

質量	濃度	體積	分子量
=	×	×

稀釋計算器分子量計算器

動物體內配方計算器（澄清溶液）

第一步：請輸入基本實驗信息（考慮到實驗過程中的損耗，建議多配一只動物的藥量）

給藥劑量 mg/kg 動物平均體重 g 每只動物給藥體積 μL 動物數量只

第二步：請輸入動物體內配方組成（配方適用于不溶于水的藥物；不同批次藥物配方比例不同，請聯(lián)系Selleck為您提供正確的澄清溶液配方）

% DMSO + % + % Tween 80 + % ddH2O

%DMSO+ %

計算結果：

工作液濃度： mg/ml；

DMSO母液配制方法： mg 藥物溶于μL DMSO溶液（母液濃度mg/mL，注：如該濃度超過該批次藥物DMSO溶解度，請先聯(lián)系Selleck）；

體內配方配制方法：取μL DMSO母液，加入μL PEG300，混勻澄清后加入μL Tween 80，混勻澄清后加入μL ddH₂O，混勻澄清。

體內配方配制方法：取μL DMSO母液，加入μL Corn oil，混勻澄清。

注意：1. 首先保證母液是澄清的；
2.一定要按照順序依次將溶劑加入，進行下一步操作之前必須保證上一步操作得到的是澄清的溶液，可采用渦旋、超聲或水浴加熱等物理方法助溶。

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Hydrocortisone (Cortisol)

產品質控

Hydrocortisone (Cortisol)相關產品

細胞實驗數據示例

生物活性

化學信息&溶解度

實驗計算

技術支持