Mycoplasmas are the smallest prokaryotic microorganism, only 0.1-0.3 μm in size. Due to their small size, mycoplasmas can penetrate rated filters (0.22~0.45 μm). Mycoplasma contamination remains a major problem in cell culture. Mycoplasmas can alter the DNA, RNA, and protein synthesis of culture cells, but they may not noticeably affect cell growth rates in many cases. Therefore, it is difficult to discover mycoplasma contamination with the naked eye. And it is a must to detect mycoplasma contamination routinely for laboratories that use cell culture frequently.

BM-Cyclin consists of tiamulin fumarate and minocycline hydrochloride, two antibiotics that both prevent protein synthesis. BM-Cyclin can inhibit and eliminate many types of mycoplasma contamination in cell culture, such as Mycoplasma orale、Mycoplasma arginine, and Mycoplasma hyorhinis, without marked cytotoxic side effects. Meanwhile, BM-Cyclin is also effective against some common bacteria.