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α-Ketoglutarate Assay Kit

Catalog No.
K2216
 
Grouped product items
SizePriceStock Qty
100assays
$95.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

α-Ketoglutarate (α-KG) is a central metabolic intermediate, functioning both in the TCA cycle and as an amino-group acceptor in amino acid synthesis. Its concentration reflects cellular metabolic status, making its quantification vital for studies in energy metabolism, physiology, and disease biomarker development.

The α-Ketoglutarate Assay Kit provides a simple, sensitive, and robust method for quantifying α-ketoglutarate in serum, plasma, cells, and tissue lysates. In the assay, α-KG reacts with alanine via alanine aminotransferase (ALT) to generate pyruvate, which is oxidized by pyruvate oxidase (PO) to produce hydrogen peroxide (H2O2). In the presence of horseradish peroxidase, the generated H2O2 reacts with the Amplex Red probe to produce resorufin, a highly fluorescent red product. The fluorescence intensity or absorbance of resorufin is directly proportional to the α-KG concentration in the sample.?

Figure 1. Typical α-KG standard curve using colorimetric reading (left) and fluorometric reading (right).

Quality Control

Quality Control & DataSheet

View current batch:
 

Components and Storage

Components

100 Assays

Storage

Lysis?Buffer

20 mL

-20°C

α-KG Assay?Buffer

20 mL

-20°C

Amplex Red

200 μL

-20°C away from light

Enzyme A

200 μL

-20°C

Enzyme B

200 μL

-20°C away from light

Cofactor

400 μL

-20°C away from light

α-KG (10 mM)

200 μL

-20°C

Shipping: ??Blue ice ????????????????????????Shelf life: 1 year

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