6-Chloro-3-indolyl-beta-D-galactopyranoside
Catalogue Number BIMB1026
Synonym(s): Salmon-Gal
6-Chloro-3-indolyl-beta-D-galactopyranoside (CAS Number: 138182-21-5) is a compound commonly used as a substrate for cell analysis.
It yields a rose-coloured precipitate, which is why it is referred to as Salmon-Gal. It can be used as an enzyme substrate in diagnostic reagents. 6-Chloro-3-i... Read More
- CAS Number
138182-21-5
- Commodity Code
2940000080
- MDL Number
MFCD01310928
- Purity
- >98%
- Notes
- Salmon-Gal is an alternative chromogenic substrate for b-D-Galactisidase. Salmon-Gal is used in conjunctionwith IPTG for detection of b-Galactosidase activityin bacterial colonies
- Formula Weight
- 329.73 g/mol
- Melting Point
- 188-190 °C
- MSDS
- Typical Batch COA
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Product Description
6-Chloro-3-indolyl-beta-D-galactopyranoside (CAS Number: 138182-21-5) is a compound commonly used as a substrate for cell analysis.
It yields a rose-coloured precipitate, which is why it is referred to as Salmon-Gal. It can be used as an enzyme substrate in diagnostic reagents. 6-Chloro-3-indolyl-beta-D-galactopyranoside is often used together with IPTG to derect beta-galactopyranoside activity in bacterial colonies. It is helpful to detect recombinants from non-recombinants, as it makes recombinants appear as salmon-coloured and non-recombinants as white. Nitroblue Tetrazolium Salt (NBT) can speed up this process yielding fast and clear results.
It can be used in conjuction with X-Glu (5-bromo-4-chloro-3-indolyl-beta-D-glucoside) for the detection of GUS and Lac activities on the same plate.
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