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B-7400 - 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, sodium salt trihydrate

B-7400

5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, sodium salt trihydrate

Chromogenic substrate for beta-glucuronidase (GUS) yielding blue precipitate upon cleavage. Used for identification of beta-glucuronidase. X-beta-D-glucuronide has been reported as chromogenic substrate in a number of chromogenic E. coli/ colifo... Read More
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  Strictly for Research Purposes only. Not for Personal or Veterinary use.

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CAS No
[370100-64-4]
Synonyms
X-beta-D-glucuronide sodium salt
EB03171
Product Code
B-7400
MDL No
MFCD00135782
MOL file
Chemical Formula
C14H18BrClNNaO10
Molecular Weight
498.65 g/mol
Smiles
C1=CC(=C(C2=C1NC=C2O[C@H]3[C@@H]([C@H]([C@@H]([C@H](O3)C(=O)[O-])O)O)O)Cl)Br.[Na+]
Long Term Storage
store at <-15°C, protect from light
Storage
store at <-15°C, protect from light
Precautionary Statement Code
P261, P262
Harmonized Tariff Codes
Switzerland: 29389000 - USA: 2938900000 - Slovakia: 2938909090 -

Chromogenic substrate for beta-glucuronidase (GUS) yielding blue precipitate upon cleavage. Used for identification of beta-glucuronidase. X-beta-D-glucuronide has been reported as chromogenic substrate in a number of chromogenic E. coli/ coliform culture media. About 95% of common E. coli possess beta-D-glucuronidase activity. Such chromogenic culture media are used for detection and enumeration of E. coli in food samples such as meat, dairy products and shellfish and have clinical applications in the assessment of urinary infections. It has gained international acceptance as highly accurate test for the presence of E. coli in drinking water samples. It is also used for the detection of the expression of GUS (E. coli beta-glucuronidase) gene fusion marker constructs in plants. The sodium salt has advantages over the cyclohexylammonium salt as being non-electrostatic and possessing a naturally-occurring cation.

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