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  1. Metabolic Enzyme/Protease
  2. Phospholipase MAGL
  3. 7-Hydroxycoumarinyl arachidonate

7-Hydroxycoumarinyl arachidonate  (Synonyms: 7-HCA; Umbelliferyl Arachidonate; 7-HC-arachidonate)

目錄號: HY-116141
一鍵復(fù)制產(chǎn)品信息
純度: 99.19%
COA 產(chǎn)品使用指南 技術(shù)支持

7-Hydroxycoumarinyl arachidonate (7-HCA) 是胞質(zhì)磷脂酶 A2 (PLA2) 的熒光底物,也是單酰基甘油脂肪酶 (MAGL) 的熒光底物。MAGL 蛋白催化 7-Hydroxycoumarinyl arachidonate 的水解,生成花生四烯酸 (AA;HY-109590) 和高熒光的 7-羥基香豆素 (7-HC;HY-N0573),7-HC 的釋放可以用熒光計進(jìn)行測量。

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7-Hydroxycoumarinyl arachidonate

7-Hydroxycoumarinyl arachidonate Chemical Structure

CAS No. : 161180-11-6

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規(guī)格 價格 是否有貨 數(shù)量
5 mg (55.73 mM * 200 μL in Ethanol) ¥800
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10 mg (55.73 mM * 400 μL in Ethanol) ¥1280
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查看 Phospholipase 亞型特異性產(chǎn)品:

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

7-Hydroxycoumarinyl arachidonate (7-HCA) is a fluorogenic substrate of cytosolic phospholipase A2 (PLA2). 7-Hydroxycoumarinyl arachidonate is also a fluorogenic substrate for monoacylglycerol lipase (MAGL). MAGL protein catalyzes the hydrolysis of 7-Hydroxycoumarinyl arachidonat to generate Arachidonic acid (AA) and the highly fluorescent 7-hydroxyl coumarin (7-HC; HY-N0573). Release of 7-HC can be measured using a fluorometer[1][2][3].

體外研究
(In Vitro)

MAGL catalyzes the hydrolysis of 7-Hydroxycoumarinyl arachidonate (7-HCA) into arachidonic acid (AA) and 7-HC. Release of 7-HC, which is fluorescent, is monitored over time with excitation (Ex) at 355 nm and emission (Em) at 460 nm[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

448.59

Formula

C29H36O4

CAS 號
性狀

液體

顏色

Colorless to light yellow

運輸條件

Room temperature in continental US; may vary elsewhere.

儲存方式

Solution, -20°C, 2 years

純度 & 產(chǎn)品資料

純度: 99.19%

參考文獻(xiàn)

7-Hydroxycoumarinyl arachidonate 相關(guān)分類

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  • 稀釋計算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量   濃度   體積   分子量 *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start) × 體積 (start) = 濃度 (final) × 體積 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱:
7-Hydroxycoumarinyl arachidonate
目錄號:
HY-116141
需求量:
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