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  1. Apoptosis
  2. Apoptosis
  3. Mas7

Mas7 (Mastoparan 7) 是 mastoparan 的結(jié)構(gòu)類(lèi)似物,是異源三聚體 Gi 蛋白及其下游效應(yīng)物的活化劑。

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Custom Peptide Synthesis

Mas7

Mas7 Chemical Structure

CAS No. : 145854-59-7

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  • 生物活性

  • 實(shí)驗(yàn)參考方法

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

Mas7 (Mastoparan 7), a structural analogue of mastoparan, is an activator of heterotrimeric Gi proteins and its downstream effectors.

體外研究
(In Vitro)

Mas7 (Mastoparan 7) produces several biological effects in different cell types. The effect of Mas7 on endogenous mono-ADP-ribosyltransferase activity is in the micromolar range with a maximal activation of 205% over the basal. In pertussis treated plasma membranes, it is found that the effect of Mas7 on endogenous mono-ADP-ribosyltransferase is partially blocked, which suggests the involvement of G-proteins, such as Gi or G0[1].
Mas7 is a basic tetradecapeptide isolated from isp venom, which activates guanine nucleotide-binding regulatory proteins (G-proteins) and stimulates apoptosis. In smooth muscle cells, Mas7 leads to an increase in the perfusion pressure. Vascular contraction is induced by Mas7. The vasoconstriction triggered by mas-7 exhibited a slower increase compared to that simulated by phenylephrine or vasopressin[2].
Exposure of hippocampal neurons to a low dose of Mas-7 increases dendritic spine density and spine head width in a time-dependent manner. Additionally, Mas-7 enhances postsynaptic density protein-95 (PSD-95) clustering in neurites and activates Gαo signaling, increasing the intracellular Ca2+ concentration[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1421.81

Formula

C67H124N18O15

CAS 號(hào)
性狀

固體

顏色

White to off-white

Sequence

Ile-Asn-Leu-Lys-Ala-Leu-Ala-Ala-Leu-Ala-Lys-Ala-Leu-Leu-NH2

Sequence Shortening

INLKALAALAKALL-NH2

運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.

儲(chǔ)存方式

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

純度 & 產(chǎn)品資料
參考文獻(xiàn)
Cell Assay
[1]

Hippocampal neurons cultured in round 35?mm coverslips at a density of 160,000 cells/coverslip are transfected with EGFP at 11 DIV. Then, at 14 DIV the neurons are placed in the imaging chamber in an isotonic solution. The EGFP-positive neurons are imaged with microscope every 5?min for 45?min after the treatment with 1?μM Mas-7. The images are processed and analyzed using ImageJ software[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

參考文獻(xiàn)
  • 摩爾計(jì)算器

  • 稀釋計(jì)算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量   濃度   體積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start) × 體積 (start) = 濃度 (final) × 體積 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱(chēng):
Mas7
目錄號(hào):
HY-P0258
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