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  2. Neuronal Signaling
  3. Amyloid-β
  4. β-Amyloid (22-35)

β-Amyloid 22-35 (Amyloid β-Protein 22-35)，β-淀粉樣蛋白的 22-35 殘基片段，對(duì)無(wú)血清培養(yǎng)的大鼠海馬神經(jīng)元具有細(xì)胞毒性作用。β-Amyloid 22-35 在中性緩沖液中形成聚集體和典型的淀粉樣纖維，類(lèi)似于 β-淀粉樣蛋白。

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Custom Peptide Synthesis

β-Amyloid (22-35)

β-Amyloid (22-35) Chemical Structure

CAS No. : 144189-71-9

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β-Amyloid (22-35) 的其他形式現(xiàn)貨產(chǎn)品:

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Other Forms of β-Amyloid (22-35):

  • 生物活性

  • 實(shí)驗(yàn)參考方法

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

β-Amyloid 22-35 (Amyloid β-Protein 22-35), the residues 22-35 fragment ofβ-amyloid protein, has a cytotoxic effect on cultured neurons from the rat hippocampus in serum-free medium. β-Amyloid 22-35 forms aggregates and typical amyloid fibrils resembling those of the β-amyloid protein in neutral buffer solution)[1].

IC50 & Target

Amyloid-β[1]
體外研究
(In Vitro)

β-Amyloid 22-35 shows significant cytotoxicity on the rat hippocampal neurons at 40 μg/mL, but exhibits no cytotoxic effect on the glial cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1403.62

Formula

C59H102N16O21S
CAS 號(hào)
Sequence

Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met
Sequence Shortening

EDVGSNKGAIIGLM
運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.
儲(chǔ)存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
純度 & 產(chǎn)品資料
參考文獻(xiàn)
Cell Assay
[1]

Hippocampal cells are cultured in DMEM supplemented with 10% (v/v) precolostrum newborn calf serum in poly-D-lysine-coated 24-well culture plates at 37°C under a gas mixture of 95% air/5% CO2. After 6 days of culture, non-neuronal cell division is halted by 24 h exposure to 10-5 cytosine arabinoside. After washing cell layers with DMEM, cells are cultured with 1 mL of DMEM per well in the absence of serum for 5 days before experiment. Cells are treated for 3 days with β-Amyloid (22-35) or its amidated (C-terminus) derivative by addition of 20 μL of stock solution (2 mg/mL of either in betaine buffer, pH 8.5, consisting of 0.7% betaine and 20 mM NH4HCO3) to 1.0 mL medium, unless otherwise specified. Control cells are treated with the betaine buffer alone. Morphological change of cells is checked throughout the course of experiment with a phase-contrast microscope. Dead cells are checked by trypan blue staining. Cell injury is assessed by measuring lactate dehydrogenase (LDH) activity released into medium during 3 days of treatment of neurons with peptides[1]
.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
參考文獻(xiàn)

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產(chǎn)品名稱(chēng)：
β-Amyloid (22-35)
目錄號(hào)：
HY-P1474
需求量：
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