文章標(biāo)題：Mitogen-Activated Protein Kinases 3/6 Reduce Auxin Signaling via Stabilizing Indoleacetic Acid-Induced Proteins 8/9 in Plant Abiotic Stress Adaptation
影響因子：4.932
期刊：INTERNATIONAL IMMUNOPHARMACOLOGY
作者列表：Chunyan Wang, Xiaoxuan Li, Han Zhao, Xiankui Cui, Wenhong Xu, Ke Li, Yang Xu, Zipeng Yu, Luyao Yu, Rui Guo
發(fā)表時(shí)間：2025-2-24
DOI:10.3390/ijms26051964
主要研究成果：first_pagesettingsOrder Article Reprints
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Mitogen-Activated Protein Kinases 3/6 Reduce Auxin Signaling via Stabilizing Indoleacetic Acid-Induced Proteins 8/9 in Plant Abiotic Stress Adaptation
by Chunyan Wang 1,Xiaoxuan Li 1,Han Zhao 1,Xiankui Cui 1,Wenhong Xu 1,Ke Li 2ORCID,Yang Xu 3ORCID,Zipeng Yu 1,Luyao Yu 1,*ORCID andRui Guo 1,*
1
The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao 266237, China
2
Shandong Academy of Grape, Shandong Academy of Agricultural Sciences, Jinan 250100, China
3
Shandong Peanut Research Institute, Shandong Academy of Agricultural Sciences, Qingdao 266100, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2025, 26(5), 1964; https://doi.org/10.3390/ijms26051964
Submission received: 15 January 2025 / Revised: 11 February 2025 / Accepted: 20 February 2025 / Published: 24 February 2025
(This article belongs to the Special Issue New Insights into Environmental Stresses and Plants)
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Abstract
The balance between plant growth and stress response is a key issue in the field of biology. In this process, mitogen-activated protein kinase 3 (MPK3) and MPK6 contribute to the construction of plants’ defense system during stress tolerance, while auxin, a growth-promoting hormone, is the key to maintaining plant growth. Nevertheless, the antagonistic or cooperative relationship between MPK3/6-mediated stress response and auxin-mediated plant growth remains unclear. Here, we demonstrate that stress-activated MPK3/6 interact with the auxin signaling repressors indoleacetic acid-induced protein 8 (IAA8) and IAA9, two key targets for regulating the auxin signaling output during stress responses. Protein phosphorylation mass spectrometry followed by a co-analysis with in vitro phosphorylation experiments revealed that MPK3/6 phosphorylated the S91, T94, and S152 residues of IAA8 and the S88 residue of IAA9. Phosphorylation significantly enhanced the protein stability of IAA8/9, thereby maintaining basal auxin signaling during plant stress adaptation. Collectively, MPK3/6-IAA8/9 are key modules that are turned on during plant stress adaptation to precisely reduce auxin signaling output, thereby preventing plants from improper vigorous growth under stress conditions.