文章標(biāo)題：Circ_0011232 contributes to hepatocellular carcinoma progression through miR-503-5p/AKT3 axis
影響因子：4.942
期刊：HEPATOLOGY RESEARCH
作者列表：Andong Ju, Yuhou Shen, Aimin Yue
發(fā)表時間：2022-2-20
DOI:10.1111/hepr.13758
主要研究成果：Abstract
Background
Hepatocellular carcinoma (HCC) is a type of primary liver cancer with high mortality. Circular RNAs (circRNAs) have been confirmed to be involved in the development of HCC, but the functions of circ_0011232 in HCC remain ill-defined.
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Methods
Quantitative real-time polymerase chain reaction, western blot assay, or immunohistochemistry assay was performed to determine the levels of circ_0011232, miR-503-5p, and AKT3. RNase R assay and actinomycin D assay were conducted to analyze the feature of circ_0011232. Cell Counting Kit-8 assay, 5-ethynyl-2′-deoxyuridine assay, colony formation assay, flow cytometry analysis, wound-healing assay, and transwell assay were conducted to evaluate HCC cell proliferation, colony formation, apoptosis, migration, and invasion. Dual-luciferase reporter assay was carried out to confirm the relationships among circ_0011232, miR-503-5p, and AKT3. The murine xenograft assay was conducted to verify the function of circ_0011232 in tumor growth in vivo.