鏈接:https://www.abinscience.cn/product/34/16199.html
名稱:Anti-HBV S/L-HBsAg/L glycoprotein Recombinant Antibody (G10)
貨號:VK539033
種屬反應性:Hepatitis B virus
應用:FCM, SPR
宿主:Human
同種型:IgG1
克隆號:G10
克隆類型:Monoclonal
靶標:Large envelope protein S, L glycoprotein, L-HBsAg, LHB, Large surface protein, Major surface protein, preS1 domain, Pre-S1
內毒素水平:Please contact with the lab for this information.
純度:>95% as determined by SDS-PAGE.
純化方式:Protein A/G purified from cell culture supernatant.
Accession號:P03141
狀態(tài):Liquid
用途范圍:僅用于科研
儲存:Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
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產品介紹:Anti-HBV S/L-HBsAg/L glycoprotein Recombinant Antibody (G10)|VK539033 是艾必賽生物(abinScience)推出的人源單克隆抗體(人 IgG1),克隆號 G10,靶向乙肝病毒(HBV)的大包膜蛋白 S / L-HBsAg / L glycoprotein,表位覆蓋 preS1 區(qū)域,可識別 LHBs 的 preS1 構象,適用于 流式細胞術(FCM) 與 表面等離子共振(SPR) 分析。該抗體經(jīng) Protein A/G 純化,SDS-PAGE 純度 >95%,以液體形式保存在 0.01M PBS, pH 7.4 中;短期 4°C(1–2 周),長期 -20°C(12 個月)/ -80°C 保存,避免反復凍融。(RUO,僅限科研/非臨床用途)
HBV 大包膜蛋白(LHBs) 是 HBV 包膜的重要組成部分,preS1 結構域(尤其是 N 端 2–48 aa)通過與肝細胞表面的?;悄懰徕c共轉運多肽(NTCP)特異性、不可逆結合,介導病毒附著—內吞—膜融合從而完成入侵,是 HBV 感染起始的關鍵步驟,也是中和抗體與入胞抑制劑(如 Bulevirtide)的核心靶點。由于 LHBs 在不同感染階段及抗病毒治療響應中的動態(tài)變化,其檢測在慢性乙肝分期、抗病毒療效預測、HBsAg 定量及中和抗體篩選中具有重要價值。G10 作為靶向 preS1/L-HBsAg 的人源 IgG1 單抗,可在流式平臺上檢測 HBV 感染模型或病毒樣顆粒表面的 HBsAg 表達,也可通過 SPR 評估其與 HBsAg/preS1 肽段的結合動力學,是 HBV 基礎研究、血清學檢測與方法學開發(fā)中的科研級參照試劑。
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參考文獻:
O-glycosylated HBsAg peptide can induce specific antibody neutralizing HBV infection. PMID: 34582939
Mechanisms underlying delayed loss of HBeAg and HBV DNA following HBsAg seroclearance in PEG-IFNalpha treated patients of chronic hepatitis B. PMID: 40035711
Characterization of HBV surface antigen isoforms in the natural history and treatment of HBV infection. PMID: 37026760
Immunogenicity of parenterally delivered plant-derived small and medium surface antigens of hepatitis B virus. PMID: 26905723
Hepatitis B virus peptide inhibitors: solution structures and interactions with the viral capsid. PMID: 26100394
A cellular protein which binds hepatitis B virus but not hepatitis B surface antigen. PMID: 10091999
Role of hepatitis B virus genotype D & its mutants in occult hepatitis B infection. PMID: 24135177
Transforming growth factor β as a possible independent factor in chronic hepatitis B. PMID: 33871695
Analysis of the cytosolic domains of the hepatitis B virus envelope proteins for their function in viral particle assembly and infectivity. PMID: 17020942
Bulevirtide monotherapy for 48 weeks in patients with HDV-related compensated cirrhosis and clinically significant portal hypertension. PMID: 35973578
Incidence of hepatitis B virus infection in young Chinese blood donors born after mandatory implementation of neonatal hepatitis B vaccination nationwide. PMID: 29624818
Plant expression, lyophilisation and storage of HBV medium and large surface antigens for a prototype oral vaccine formulation. PMID: 22246107
[The relationship between serum HBV pgRNA and antigen status in patients with chronic hepatitis B after long-term nucleotide analogues treatment]. PMID: 34517458
[Use of baculovector for the expression of HBsAg gene in insect cells]. PMID: 2800542
Adsorption and penetration of hepatitis B virus in a nonpermissive cell line. PMID: 8184545
Inhibition of hepatitis B virus assembly with synthetic peptides derived from the viral surface and core antigens. PMID: 12469306
Two distinct segments of the hepatitis B virus surface antigen contribute synergistically to its association with the viral core particles. PMID: 10024452
Micropropagation of transgenic lettuce containing HBsAg as a method of mass-scale production of standardised plant material for biofarming purposes. PMID: 27655251
Peptides that block hepatitis B virus assembly: analysis by cryomicroscopy, mutagenesis and transfection. PMID: 9843489
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