? ?文章標(biāo)題：Overexpression of FTO inhibits excessive proliferation ａnd promotes the apoptosis of human glomerular mesangial cells by alleviating FOXO6 m6A modification via YTHDF3-dependent mechanisms
影響因子：5.6
期刊：Frontiers in Pharmacology
作者列表：Xingxing Zhuang, Tao Liu, Liangbing Wei, Jiarong Gao
發(fā)表時(shí)間：2023-9-26
DOI:10.3389/fphar.2023.1260300
主要研究成果：Abstract
Background: N6-methyladenosine （m6A） is a prevalent post-transcriptional modification presented in messenger RNA （mRNA） of eukaryotic organisms. Chronic glomerulonephritis （CGN） is characterised by excessive proliferation ａnd insufficient apoptosis of human glomerular mesangial cells （HGMCs） but its underlying pathogenesis remains undefined. Moreover, the role of m6A in CGN is poorly understood.
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Methods: The total level of m6A modification was detected using the m6A quantification assay （Colorimetric）. Cell proliferation was assessed by EdU cell proliferation assay, ａnd cell apoptosis was detected by flow cytometry. RNA sequencing was performed to screen the downstream target of fat mass ａnd obesity-associated protein （FTO）. MeRIP-qPCR was conducted to detect the m6A level of forkhead box o6 （FOXO6） in HGMCs. RIP assay was utilized to indicate the targeting relationship between YTH domain family 3 （YTHDF3） ａnd FOXO6. Actinomycin D assay was used to investigate the stability of FOXO6 in HGMCs.