谷光甘肽合成酶(GSS)重組蛋白
[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]
Residues: Residues: Residues: Residues: Asp268~Val474 (Accession # P48637),
with N-terminal His-Tag.
Host: E. coli
Purity: Purity: Purity: Purity: >95%
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 6.1
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 24.3kDa
Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-DGY MPRQYSLQNW EARLLLERSH AAKCPDIATQ LAGTKKVQQE
LSRPGMLEML LPGQPEAVAR LRATFAGLYS LDVGEEGDQA IAEALAAPSR FVLKPQREGG
GNNLYGEEMV QALKQLKDSE ERASYILMEK IEPEPFENCL LRPGSPARVV QCISELGIFG
VYVRQEKTLV MNKHVGHLLR TKAIEHADGG VAAGVAVLDN PYPV
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關鍵字： Glutathione;synthase;GSS;谷光甘肽合成酶(GSS)重組蛋白;
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BIOESN研究領域及主要產品 :
主要研究領域是生命科學，包括分子生物學、細胞生物學、免疫學試劑、重組蛋白，抗體，實驗技術服務等多個應用領域。主要產品有：Elisa檢測試劑盒、PCR檢測試劑盒、抗體、重組蛋白，蛋白定制，細胞系，原代細胞，常用實驗試劑，染色液等科研產品。