內(nèi)皮素2(EDN2)重組蛋白
[ PROPERTIES ]
Residues: Gln32~Arg178
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P20800
Host: E. coli
Subcellular Location: Secreted.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 10.8
Predicted Molecular Mass: 20.5kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of thetarget
protein. The loss rate was determined by accelerated thermal degradationtest,
that is, incubate the protein at 37oC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
QPASSSHAQ GTHLRLRRCS CSSWLDKECV YFCHLDIIWV NTPEQTAPYGLGNPPRRRRRSLPRRCQCSS ARDPACATFC LRRPWTEAGA VPSRKSPADV FQTGKTGATT GELLQRLRDI
STVKSLFAKR QQEAMREPRS THSRWRKR
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關(guān)鍵字： ET2;PPET2;EDN2;內(nèi)皮素2(EDN2)重組蛋白;
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