過氧化還原酶3(PRDX3)重組蛋白
[ PROPERTIES ]
Residues: Val5~Trp233
Tags: N-terminal His-Tag
Accession: P30048
Host: E. coli
Subcellular Location: Mitochondrion.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 10mM
PBS, pH7.4, containing 1mM DTT, 5% trehalose,
0.01% sarcosyl and preservative.
Predicted isoelectric point: 6.9
Predicted Molecular Mass: 26.4kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8
oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
VGRLLR ASVARHVSAI PWGISATAAL RPAACGRTSL TNLLCSGSSQ AKLFSTSSSC
HAPAVTQHAP YFKGTAVVNG EFKDLSLDDF KGKYLVLFFY PLDFTFVCPT EIVAFSDKAN
EFHDVNCEVV AVSVDSHFSH LAWINTPRKN GGLGHMNIAL LSDLTKQISR DYGVLLEGSG
LALRGLFIID PNGVIKHLSV NDLPVGRSVE ETLRLVKAFQ YVETHGEVCP ANW
[ REFERENCES ]
1. Cao Z., et al. (2007) J. Mol. Biol. 372:1022-1033.
2. Masaki M., et al. (2003) Eur. J. Biochem. 270:76-83.
3. Wagner E., et al. (2002) Biochem. J. 366:777-785.
4. Tsuji K., et al. (1995) Biochem. J. 307:377-381.
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關(guān)鍵字： MER5; AOP-1;AOP1;過氧化還原酶3(PRDX3)重組蛋白;
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