17-β-羥基類固醇脫氫酶12(HSD17b12)重組蛋白
[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Met1~Asn312
Tags: N-terminal His-Tag
Tissue Specificity: Ovary, Breast. Subcellular Location: Endoplasmic reticulum membrane; Multi-pass membraneprotein. Purity: >98%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mMEDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine ReactiveLabeling. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 9.3
Predicted Molecular Mass: 38.0kDa
Accurate Molecular Mass: 38kDa as determined by SDS-PAGE reducingconditions.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
oC for one month. Aliquot and store at -80
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. Thelossratewas determined by accelerated thermal degradation test, that is, incubatetheprotein at 37
oC for 48h, and no obvious degradation and precipitationwereobserved. The loss rate is less than 5% within the expiration dateunderappropriate storage condition.
[ SEQUENCE ]
[ IDENTIFICATION ]
Figure 1. SDS-PAGE
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關鍵字： KAR;SDR12C1;Estradiol ;17-β-羥基類固醇脫氫酶12(HSD1;
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主要研究領域是生命科學，包括分子生物學、細胞生物學、免疫學試劑、重組蛋白，抗體，實驗技術服務等多個應用領域。主要產品有：Elisa檢測試劑盒、PCR檢測試劑盒、抗體、重組蛋白，蛋白定制，細胞系，原代細胞，常用實驗試劑，染色液等科研產品。