Mouse Anti-IKB alpha Monoclonal Antibody
DATASHEET
Host:Mouse
Target Protein:IKB alpha
IR:Immunogen Range:1-77/314
Clonality:Monoclonal
Isotype:IgG1
Entrez Gene:4792
Swiss Prot:P25963
Source:Recombinant human IKB alpha Protein:1-77/314
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IKB alpha and IKB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkB and block nuclear import by masking the nuclear localisation sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IKB. Immunoblotting easily detects the hyperphosphorylated forms of IKB alpha, but not phosphorylated IKB beta. Interestingly, IKB alpha and IKB beta mediate different NFkB responses. IkB alpha appears to control more transient activation of NFkB in response to an inducer, while IKB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IKB is confirmed by immunoblotting.
Size:50ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
僅供科研使用。不能用于人和動(dòng)物治療等其它臨床診斷用！
關(guān)鍵字： IKB alpha;NFKBIA; Inhibitor of KB alp;I(Kappa)B(alpha);IKBA;IKBalpha;MAD3;IKBA_HUMAN;
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