Recombinant Human Glutamate Cysteine Ligase, Catalytic (GCLC)
Sequence Information
Species：Human Gene ID:2729
Swiss Prot:P48506 Synonyms: GCS; GLCL; GLCLC
Residues:Met429-Asn637
MEVQLTDFENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQKRDAVLQGMFYFRKDICKGGNAVVDGCGKAQNSTELAAEEYTLMSIDTIINGKEGVFPGLIPILNSYLENMEVDVDTRCSILNYLKLIKKRASGELMTVARWMREFIANHPDYKQDSVITDEMNYSLILKCNQIANELCECPELLGSAFRKVKYSGSKTDSSN
Product Information
Source: Prokaryotic expression. Host: E. coli
Tags: N-terminal His-Tag
Subcellular Location: Secreted. Purity: >90%
Traits: Freeze-dried powder
Buffer formulation: PBS（PH7.4)）, containing 5% Trehalose.
Original Concentration: 400μg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 5.3
Predicted Molecular Mass: 25.5kDa
Accurate Molecular Mass: 25kDa as determined by SDS-PAGE reducing conditions.
[USAGE ]
Reconstitute in PBS (pH7.4) to a concentration of 0.1-0.5 mg/mL. Do not vortex.
[STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8℃ for one month.
Aliquot and store at -80℃ for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate wasdetermined by accelerated thermal degradation test, that is, incubate the protein at 37℃for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 5% within the expiration date under appropriate storage condition.
[IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for in vitro and research use only, we will not be responsible for anyissue if the kit was used in clinical diagnostic or any other procedures.
僅供科研使用。不能用于人和動(dòng)物治療等其它臨床診斷用！
關(guān)鍵字： GCS;GLCL;GLCLC;Recombinant ;Human ;Glutamate ;Cysteine ;Ligase;
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