分子名稱：Spike protein
英文名稱：Coronavirus spike glycoprotein
中文名稱：冠狀病毒刺突糖蛋白
靶點(diǎn)別稱：Spike,S protein,Spike glycoprotein,S glycoprotein
物種：人/鼠/猴等
屬性：重組蛋白/抗體/試劑盒/磁珠/假病毒
標(biāo)記：Biotin-labeled / Unconjugated
表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）
SARS-CoV-2 S protein, His Tag, Super stable trimer (SPN-C52H9) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 ). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
Predicted N-terminus: Val 16
蛋白結(jié)構(gòu)（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 138.0 kDa. The protein migrates as 150-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
內(nèi)毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
純度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制劑（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS with trehalose as protectant.
Contact us for customized product form or formulation.
重構(gòu)方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.
背景（Background）
據(jù)報(bào)道，SARS-CoV-2可通過與人ACE2受體的相互作用感染人類呼吸道上皮細(xì)胞。Spike protein是一種大的I型跨膜蛋白，包含兩個亞基，S1和S2。S1主要包含一個受體結(jié)合結(jié)構(gòu)域（RBD），負(fù)責(zé)識別細(xì)胞表面受體。S2包含膜融合所需的基本元素。S蛋白在誘導(dǎo)中和抗體和T細(xì)胞反應(yīng)以及保護(hù)性免疫中起著關(guān)鍵作用。
用戶評價(jià)
關(guān)鍵字： Spike protein;Spike蛋白;冠狀病毒刺突糖蛋白 ;新冠糖蛋白;S protein;
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