resDetect? DNase Activity Assay Kit (Fluorescence)

背景（Background）

Deoxyribonucleases (DNases) are enzymes which are able to hydrolyze phosphodiester bonds of DNA molecules. They can be divided into two families, which differ in biochemical and biological properties—DNase I and DNase II families. The presence of DNases would affect many experimental results like PCR, so it is necessary to evaluate the presence of DNases.

DNases are ubiquitous in both the environment and many biological materials. Many molecular biology experiments rely on the use of plastics, chemicals, and solutions that are free from detectable DNase activity, once these materials are contaminated with DNases, the experiments will be affected because of the DNases can degrade DNA. Since even only minute amounts of DNase contamination would ruin the experiment, it is necessary to evaluate the presence of DNase with a reasonable method.

Published methods for detecting DNase such as Nucleic acid hydrolyzed gel electrophoresis and ultraviolet spectrophotometer are typically time consuming, not quantitative, and relatively insensitive. The other methods such as HPLC and electrochemical methods are limited to instrumentation.

In contrast, the DNase Activity Assay Kit (Fluorescence) can detect DNase contamination within 30 minutes, and the kit is high sensitivity, easy to use. Moreover, the DNase and RNase Activity Assay Kit (Fluorescence) have been designed to work together seamlessly for simultaneous quantitative detection of DNases and RNases in a single sample.

應(yīng)用說明（Application）

The DNase Activity Assay Kit (Fluorescence) is a convenient and sensitive assay tool to test the presence of DNase in buffers, reagents, and other components.

It is for research use only.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存儲（Storage）

The unopened kit should be stored at -20°C upon receiving.

Find the expiration date on the outside packaging and do not use reagents past their expiration date.

The opened kit should be stored per components table. The shelf life is 3 months from the date of opening.

原理（Assay Principles）

The DNase Activity Assay Kit (Fluorescence) is based on a fluorophore-labeled DNase substrate. When the sample does not contain DNase activity, the substrate is stable and does not produce a fluorescent signal; when the sample contains DNase activity, the substrate is degraded, resulting in a gradual enhanced fluorescence signal, the rate of increase in fluorescence signal is positively correlated with the number and activity of enzymes. Use a fluorescence microplate reader to measure at the wavelength of ex/em = 535/565 nm to determine whether the sample is contaminated by DNase.