基本信息
產(chǎn)品名稱：Goat Anti-Mouse IgG H&L Antibody
規(guī)格或純度：無載體, ExactAb?, 已驗(yàn)證, 見COA
應(yīng)用：ELISA, LF, GICA
應(yīng)用：ELISA,LF,GICA
種屬反應(yīng)性：小鼠(Mouse)
交叉反應(yīng)：No cross reactivity with Rabbit IgG and Human IgG.
免疫原：Mouse IgG
陽性對(duì)照：Mouse IgG
偶聯(lián)：Unconjugated
產(chǎn)品屬性
抗體類型：secondary antibody
克隆類型：多克隆抗體
Format：Whole IgG
SDS-PAGE：150kDa
純化方法：Antigen affinity purified
來源：Serum
物理外觀：Liquid
儲(chǔ)存緩沖液：10mM PB, 150mM NaCl, 0.09% Sodium azide, pH7.4
防腐劑：0.09% Sodium azide
濃度：見COA
儲(chǔ)存溫度：-20°C儲(chǔ)存,避免反復(fù)凍融
運(yùn)輸條件：超低溫運(yùn)輸
穩(wěn)定性與儲(chǔ)存：長(zhǎng)期儲(chǔ)存-20℃（36個(gè)月）；收貨后建議分裝，避免反復(fù)凍融。
功能和特點(diǎn)
宿主種屬：山羊(Goat)
種屬反應(yīng)性：小鼠(Mouse)
偶聯(lián)：Unconjugated
圖片
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IF: Goat Anti-Mouse IgG H&L Antibody (Ab115835)
Goat Anti-Mouse IgG H&L Antibody (Ab115835) - IF
IF analysis of beta Tubulin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L Antibody (Ab115835) conjugated with biotin at a dilution of 1/1000 for 1 hour at room temperature in the dark. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at a dilution of 1/200 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
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Flow Cytometry: Goat Anti-Mouse IgG H&L Antibody (Ab115835)
Goat Anti-Mouse IgG H&L Antibody (Ab115835) - Flow Cytometry
Flow analysis of beta Actin (red line) in Jurkat cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes. Cells were stained with beta Actin Mouse mAb (Ab155822) at 1/5000 dilution for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L Antibody (Ab115835) conjugated with FITC (Fluorescein isothiocyanate) at a dilution of 1/1000 for 30 minutes at 4°C in the dark . Unlabeled sample (black line) was used as a negative control.
查看阿拉丁官網(wǎng)此產(chǎn)品相關(guān)對(duì)應(yīng)頁面：https://www.aladdin-e.com/zh_cn/Ab115835.html
關(guān)鍵字： 山羊抗小鼠IgG抗體 ; 山羊抗小鼠IgG ; 山羊抗鼠IgG 抗體 ; 山羊抗鼠IgG
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