基本描述
產(chǎn)品名稱:Recombinant Human IL-2 Protein
規(guī)格或純度:無載體,生物活性,ActiBioPure?,無疊氮鈉,高性能,His標(biāo)簽,≥95%(SDS-PAGE)
產(chǎn)品介紹:
白介素2即白細(xì)胞介素-2(interleukin-2,IL-2),又名T細(xì)胞生長因子(T cell growth factor,TCRF)。主要由活化的CD4+T細(xì)胞和CD8+T細(xì)胞產(chǎn)生的具有廣泛生物活性的細(xì)胞因子。是所有T細(xì)胞亞群的生長因子,能使T細(xì)胞在試管內(nèi)長期存活,并可促進(jìn)活化B細(xì)胞增殖,故為調(diào)控免疫應(yīng)答的重要因子,也參與抗體反應(yīng)、造血和腫瘤監(jiān)視。?
本產(chǎn)品由真核細(xì)胞表達(dá)的重組蛋白,是將蛋白基因接入穩(wěn)定表達(dá)載體,轉(zhuǎn)化CHO-K1細(xì)胞,經(jīng)克隆篩選,質(zhì)量驗證,挑選的穩(wěn)定表達(dá)株,由化學(xué)成分確定的培養(yǎng)基(不含有任何動物源成分)發(fā)酵制備,工藝穩(wěn)定,保證了產(chǎn)品質(zhì)量及批次間的一致性。
本產(chǎn)品為無菌凍干粉劑,由含有 10mM 磷酸鹽pH為 7.2的蛋白溶液經(jīng)0.2μm過濾后分裝凍干。
生物活性:1、Measured in a cell proliferation assay using CTLL?2 mouse cytotoxic T cells. The ED50 for this effect is typically less than 2.5 ng/mL. 2、Measured in a cell proliferation assay using mouse splenocytes. The ED50 for this effect is typically less than 30.0 ng/mL.
純度:≥95% (SDS-PAGE, SEC-HPLC)
氨基酸:21-153 aa
蛋白長度:Full length protein
無載體:Yes
Accession #:P60568
預(yù)測分子量:15.4 kDa
SDS-PAGE:13.0 kDa, under reducing conditions; 13.0 kDa, under non-reducing conditions.
級別:無載體, 生物活性, ActiBioPure?, 無疊氮鈉, 高性能, His標(biāo)簽
產(chǎn)品規(guī)格參數(shù)
儲存緩沖液:含有 10mM 磷酸鹽pH為 7.2的蛋白溶液經(jīng)0.2μm過濾后分裝凍干
復(fù)溶:建議將凍干 rhIL-2溶解在注射用水或滅菌的超純水中,濃度不低于100μg/ml,以待進(jìn)一步稀釋至工作濃度。盡可能避免反復(fù)凍融。 復(fù)溶后的細(xì)胞因子:4℃可穩(wěn)定儲存7-10天;短期保存請分裝后存放于-20℃。
儲存溫度:-20°C儲存
運(yùn)輸條件:超低溫運(yùn)輸
穩(wěn)定性與儲存:發(fā)貨時溫度為 4°C。儲存溫度為 -20°C。凍干,無添加劑。本品為活性蛋白質(zhì),可能會引起體內(nèi)生物反應(yīng),請謹(jǐn)慎處理。
功能和特點
Accession #:P60568
生物活性:1、Measured in a cell proliferation assay using CTLL?2 mouse cytotoxic T cells. The ED50 for this effect is typically less than 2.5 ng/mL. 2、Measured in a cell proliferation assay using mouse splenocytes. The ED50 for this effect is typically less than 30.0 ng/mL.
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Protein Bioactivity: Recombinant Human IL-2 Protein (rp147476)
Recombinant Human IL-2 Protein (rp147476)-Protein Bioactivity
Measured in a cell proliferation assay using CTLL?2 mouse cytotoxic T cells. The ED??
for this effect is typically <2.5ng/mL.

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SDS-PAGE: Recombinant Human IL-2 Protein (rp147476)
Recombinant Human IL-2 Protein (rp147476) -SDS-PAGE
3μg/lane of Recombinant Human IL-2 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie? Blue staining, showing a single band at 13.0 kDa.

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Protein Bioactivity: Recombinant Human IL-2 Protein (rp147476)
Recombinant Human IL-2 Protein (rp147476) - Protein Bioactivity
Measured in a cell proliferation assay using mouse splenocytes. The ED50 for this effect is typically less than 30.0 ng/mL.?
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關(guān)鍵字: 重組人白細(xì)胞介素-2蛋白 ; 重組人 IL-2 蛋白;IL-2 ; T-cell growth factor
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