基本信息
產(chǎn)品名稱：Recombinant FGF2 Antibody
規(guī)格或純度：ExactAb?, Validated, Recombinant, High performance, 0.103 mg/mL
應用：WB, IF/ICC, IP, ELISA, IHC
特異性：FGF2
種屬反應性：人(Human)
免疫原：Synthetic peptide within Human FGF2 (AA: 100-200).
陽性對照：WB: HeLa, A549 and HUVEC cell lysates. IHC: Human prostate cancer tissue. IF/ICC: HeLa cells.
偶聯(lián)：Unconjugated
產(chǎn)品屬性
克隆類型：重組抗體
克隆號：EP1735
Format：Whole IgG
亞型：Rabbit IgG
SDS-PAGE：150 kDa
純化方法：Protein A purified
物理外觀：Liquid
儲存緩沖液：59% 10mM PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.2
防腐劑：0.01% Sodium azide
濃度：0.103 mg/mL
儲存溫度：-20°C儲存,避免反復凍融
運輸條件：超低溫運輸
穩(wěn)定性與儲存：Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
功能和特點
宿主種屬：兔(Rabbit)
種屬反應性：人(Human)
克隆號：EP1735
偶聯(lián)：Unconjugated
圖片
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Western Blot: Recombinant FGF2 Antibody (Ab007066)
Recombinant FGF2 Antibody (Ab007066) - Western Blot
All lanes: Recombinant FGF2 Antibody (Ab007066) at 1/1000 dilution
Samples: Lysates at 20 μg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 18, 20, 22, 24 kDa
Observed band size: 18,2 2, 25 kDa
Exposure time: 8.0 s
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IHC: Recombinant FGF2 Antibody (Ab007066)
Recombinant FGF2 Antibody (Ab007066) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue sections labeling FGF2 with purified Recombinant FGF2 Antibody (Ab007066). Heat mediated antigen retrieval was performed.
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IF/ICC: Recombinant FGF2 Antibody (Ab007066)
Recombinant FGF2 Antibody (Ab007066) - IF/ICC
IF analysis of Recombinant FGF2 Antibody (Ab007066) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant FGF2 Antibody (Ab007066) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (D1372407) (blue).
查看阿拉丁官網(wǎng)此產(chǎn)品相關對應頁面：https://www.aladdin-e.com/zh_cn/Ab007066.html
關鍵字： 重組FGF2抗體;Basic fibroblast growth factor antibody ; Basic fibroblast growth factor bFGF antibody ; BFGF antibody
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