基本信息
產(chǎn)品名稱：APE1 Mouse mAb
規(guī)格或純度：ExactAb?, Validated, Carrier Free, 0.5 mg/mL
應(yīng)用：WB, IF/ICC, IHC
特異性：APEX1
種屬反應(yīng)性：人(Human), 小鼠(Mouse), 大鼠(Rat)
免疫原：Recombinant protein of human APEX1 (AA 1-318).
陽性對照：WB: HeLa, LNCAP, K562, NIH/3T3 and C6 cell lysates. IHC: Human stomach tissue. ICC/IF: HeLa cells.
偶聯(lián)：Unconjugated
產(chǎn)品屬性
克隆類型：單克隆抗體
克隆號：1518CT337.123.86.269.232
Format：Whole IgG
亞型：Mouse IgG1
輕鏈亞型：kappa
SDS-PAGE：150 kDa
純化方法：Protein G purified
物理外觀：Liquid
儲存緩沖液：10mM PBS, 0.09% Sodium azide, pH7.4.
防腐劑：0.09% Sodium azide
濃度：0.5 mg/mL
儲存溫度：-20°C儲存,避免反復凍融
運輸條件：超低溫運輸
穩(wěn)定性與儲存：4°C 短期儲存（2 周）。-20°C下長期保存（12個月）。收貨后建議分裝。避免冷凍/解凍循環(huán)。
功能和特點
宿主種屬：小鼠(Mouse)
種屬反應(yīng)性：人(Human), 小鼠(Mouse), 大鼠(Rat)
克隆號：1518CT337.123.86.269.232
偶聯(lián)：Unconjugated
圖片
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Western Blot: APE1 Mouse mAb (Ab089159)
APE1 Mouse mAb (Ab089159) - Western Blot
All lanes: APE1 Mouse mAb (Ab089159) at 1/1000 dilution
Samples: Lysates at 20 μg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 35 kDa
Exposure time: 45.4 s
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IF: APE1 Mouse mAb (Ab089159)
APE1 Mouse mAb (Ab089159) - IF
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling APE1 Mouse mAb (Ab089159) at 1/25 dilution, followed by Dylight? 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight? 554 Phalloidin at 1/100 dilution (red).
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IHC: APE1 Mouse mAb (Ab089159)
APE1 Mouse mAb (Ab089159) - IHC
Immunohistochemical analysis of paraffin-embedded Human stomach section using APE1 Mouse mAb (Ab089159) at 1/50 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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IHC: APE1 Mouse mAb (Ab089159)
APE1 Mouse mAb (Ab089159) - IHC
Immunohistochemical analysis of paraffin-embedded human stomach sections labeling APE1 with APE1 Mouse mAb (Ab089159). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
查看阿拉丁官網(wǎng)此產(chǎn)品相關(guān)對應(yīng)頁面：https://www.aladdin-e.com/zh_cn/Ab089159.html
關(guān)鍵字： 多功能DNA修復酶抗體 ; 脫嘌呤/脫嘧啶核酸內(nèi)切酶1(APEX1)單克隆抗體 ; APE1小鼠單克隆抗體 ; APE1小鼠單抗
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