基本信息
產(chǎn)品名稱：Recombinant Bax Antibody
規(guī)格或純度：ExactAb?, Validated, Recombinant, High performance, 0.25mg/mL
應(yīng)用：WB, IF/ICC, IP, Flow Cytometry, IHC
特異性：Bax
種屬反應(yīng)性：人(Human), 小鼠(Mouse), 大鼠(Rat)
免疫原：A synthetic peptide of human BAX (AA 1-100).
陽性對(duì)照：WB: A549, HT-29, HeLa, LNCaP, Hep G2 and HKE293 cell lysates. IHC: Human Ovarian cancer tissue. IF/ICC: HeLa cells. Flow: HeLa cells. IP: HT-29 whole cell lysate.
偶聯(lián)：Unconjugated
備注：本產(chǎn)品即將售完下架，替代產(chǎn)品為 Ab090655
產(chǎn)品屬性
克隆類型：重組抗體
克隆號(hào)：RR647
Format：Whole IgG
亞型：Rabbit IgG
SDS-PAGE：150 kDa
純化方法：Protein A purified
物理外觀：Liquid
儲(chǔ)存緩沖液：59% 10mM PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3.
防腐劑：0.01% Sodium azide
濃度：0.25mg/mL
儲(chǔ)存溫度：-20°C儲(chǔ)存,避免反復(fù)凍融
運(yùn)輸條件：超低溫運(yùn)輸
穩(wěn)定性與儲(chǔ)存：4°C 短期儲(chǔ)存（1-2 周）。-20°C下長期保存（24個(gè)月）。收貨后建議分裝。避免冷凍/解凍循環(huán)。
功能和特點(diǎn)
宿主種屬：兔(Rabbit)
種屬反應(yīng)性：人(Human), 小鼠(Mouse), 大鼠(Rat)
克隆號(hào)：RR647
偶聯(lián)：Unconjugated
圖片
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Western Blot: Recombinant Bax Antibody (Ab090652)
Recombinant Bax Antibody (Ab090652) - Western Blot
All lanes: Recombinant Bax Antibody (Ab090652) at 1/1000 dilution
Samples: Lysates at 20 μg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 20 kDa
Exposure time: 6.2 s
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IHC: Recombinant Bax Antibody (Ab090652)
Recombinant Bax Antibody (Ab090652) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian cancer tissue labelling Bax with Recombinant Bax Antibody (Ab090652) at 1/1600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
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Flow Cytometry: Recombinant Bax Antibody (Ab090652)
Recombinant Bax Antibody (Ab090652) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant Bax Antibody (Ab090652) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant Bax Antibody (Ab090652) (1/400 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.
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IP: Recombinant Bax Antibody (Ab090652)
Recombinant Bax Antibody (Ab090652) - IP
Bax was immunoprecipitated from 0.5 mg HT-29 whole cell lysate with Recombinant Bax Antibody (Ab090652) at 1/25 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant Bax Antibody (Ab090652) IP in HT-29 whole cell lysate
Lane 2: PBS instead of Recombinant Bax Antibody (Ab090652) in HT-29 whole cell lysate
Lane 3: HT-29 whole cell lysate, 10 μg (input)
Exposure: 30 s.
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IF/ICC: Recombinant Bax Antibody (Ab090652)
Recombinant Bax Antibody (Ab090652) - IF/ICC
IF analysis of Recombinant Bax Antibody (Ab090652) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant Bax Antibody (Ab090652) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (FITC) (Ab170148) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
查看阿拉丁官網(wǎng)此產(chǎn)品相關(guān)對(duì)應(yīng)頁面：https://www.aladdin-e.com/zh_cn/Ab090652.html
關(guān)鍵字： Bax抗體 ; anti- Bax抗體 ; Bcl2關(guān)聯(lián)X蛋白抗體 ; 重組Bax抗體
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