基本信息
產(chǎn)品名稱：Recombinant Cytokeratin 20 Antibody
規(guī)格或純度：ExactAb?, Validated, Recombinant, High performance, 0.5 mg/mL
應用：WB, IF/ICC, IP, Flow Cytometry, IHC
特異性：Cytokeratin 20
種屬反應性：人(Human)
預測反應種屬：大鼠(Rat), 普通狨(Common marmoset), 山羊(Goat), 豬(Pig)
交叉反應：Human
免疫原：A synthetic peptide corresponding to residues on the C-terminus of human Cytokeratin 20.
陽性對照：WB: HT-29 cell lysate. IHC: Colonic adenocarcinoma tissue. Flow: HT-29 cells. ICC/IF: HT-29 cells. IP: HT-29 whole cell lysate.
偶聯(lián)：Unconjugated
產(chǎn)品屬性
克隆類型：重組抗體
克隆號：RR648
Format：Whole IgG
亞型：Rabbit IgG
SDS-PAGE：150 kDa
純化方法：Protein A purified
物理外觀：Liquid
儲存緩沖液：59% 10 mM PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3.
防腐劑：0.01% Sodium azide
濃度：0.5 mg/mL
儲存溫度：-20°C儲存,避免反復凍融
運輸條件：超低溫運輸
穩(wěn)定性與儲存：4°C 短期儲存（1-2 周）。-20°C下長期保存（24個月）。收貨后建議分裝。避免冷凍/解凍循環(huán)。
功能和特點
宿主種屬：兔(Rabbit)
種屬反應性：人(Human)
克隆號：RR648
偶聯(lián)：Unconjugated
圖片
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Western Blot: Recombinant Cytokeratin 20 Antibody (Ab098925)
Recombinant ACTA2 Antibody (Ab088446) - Western Blot
All lanes: Recombinant Cytokeratin 20 Antibody (Ab098925) at 1/1000 dilution
Samples: Lysates at 20 μg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 46 kDa
Exposure time: 0.1 s
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IF/ICC: Recombinant Cytokeratin 20 Antibody (Ab098925)
Recombinant Cytokeratin 20 Antibody (Ab098925) - IF/ICC
Recombinant Cytokeratin 20 Antibody (Ab098925) staining Cytokeratin 20 in HT-29 cells by IF/ICC (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant Cytokeratin 20 Antibody (Ab098925) (1/200) at 4°C. An AF488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500).
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Flow Cytometry : Recombinant Cytokeratin 20 Antibody (Ab098925)
Recombinant Cytokeratin 20 Antibody (Ab098925) - Flow Cytometry
Overlay histogram showing HT-29 cells stained with Recombinant Cytokeratin 20 Antibody (Ab098925) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant Cytokeratin 20 Antibody (Ab098925) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at 4°C. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at 4°C. Unlabeled sample (Blue) was used as a control.
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IHC: Recombinant Cytokeratin 20 Antibody (Ab098925)
Recombinant Cytokeratin 20 Antibody (Ab098925) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of colonic adenocarcinoma tissue labelling Cytokeratin 20 with Recombinant Cytokeratin 20 Antibody (Ab098925) at 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
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IP: Recombinant Cytokeratin 20 Antibody (Ab098925)
Recombinant Cytokeratin 20 Antibody (Ab098925) - IP
Cytokeratin 20 was immunoprecipitated from 0.5 mg of HT-29 lysate with Recombinant Cytokeratin 20 Antibody (Ab098925) at 1/50 dilution.
2nd Ab: G&R HRP for IP 1/500
Lane 1: Recombinant Cytokeratin 20 Antibody (Ab098925) IP in HT-29 whole cell lysate
Lane 2: PBS instead of Recombinant Cytokeratin 20 Antibody (Ab098925) in HT-29 whole cell lysate
Lane 3: HT-29 whole cell lysate, 10 μg (input)
Exposure: 30.0 s.
查看阿拉丁官網(wǎng)此產(chǎn)品相關對應頁面：https://www.aladdin-e.com/zh_cn/Ab098925.html
關鍵字： anti- Cytokeratin 20抗體 ; 細胞角蛋白20抗體 ; 重組細胞角蛋白20抗體;CD20 antibody
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