基本信息
產品名稱：Recombinant ATP1A1 Antibody
規(guī)格或純度：ExactAb?, Validated, Recombinant, High performance, 0.5 mg/mL
應用：WB, IP, Flow Cytometry, IHC
特異性：ATP1A1
種屬反應性：人(Human), 小鼠(Mouse), 大鼠(Rat)
交叉反應：Cross-reactivity with other species has not been measured.
免疫原：A synthetic peptide corresponding to residues on the N-terminus of human NaK ATPase was used as an immunogen.
陽性對照：WB: C6, HT29, A431, Hep G2, and Raw264.7 cell lysates. IHC: Endometrium tissue. Flow: HeLa cells. IP: TT whole cell lysate.
偶聯(lián)：Unconjugated
產品屬性
克隆類型：重組抗體
克隆號：RR685
Format：Whole IgG
亞型：Rabbit IgG
SDS-PAGE：150 kDa
純化方法：Protein A purified
物理外觀：Liquid
儲存緩沖液：59% 10mM PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3
防腐劑：0.01% Sodium azide
濃度：0.5 mg/mL
儲存溫度：-20°C儲存,避免反復凍融
運輸條件：超低溫運輸
穩(wěn)定性與儲存：4°C 短期儲存（1-2 周）。-20°C下長期保存（24個月）。收貨后建議分裝。避免冷凍/解凍循環(huán)。
功能和特點
宿主種屬：兔(Rabbit)
種屬反應性：人(Human), 小鼠(Mouse), 大鼠(Rat)
克隆號：RR685
偶聯(lián)：Unconjugated
圖片
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Western Blot: Recombinant ATP1A1 Antibody (Ab128631)
Recombinant ATP1A1 Antibody (Ab128631) - Western Blot
All lanes: Recombinant ATP1A1 Antibody (Ab128631) at 1/2000 dilution
Samples: Lysates at 20 μg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 100 kDa
Observed band size: 97 kDa
Exposure time: 32.2 s
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Western Blot: Recombinant ATP1A1 Antibody (Ab128631)
Recombinant ATP1A1 Antibody (Ab128631) - Western Blot
All lanes: Recombinant ATP1A1 Antibody (Ab128631) at 1/1000 dilution
Samples: Lysates at 20 μg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 100 kDa
Observed band size: 97 kDa
Exposure time: 10.0 s
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IHC: Recombinant ATP1A1 Antibody (Ab128631)
Recombinant ATP1A1 Antibody (Ab128631) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of endometrium tissue labelling NaK ATPase with Recombinant ATP1A1 Antibody (Ab128631) at 1/10000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
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Flow Cytometry: Recombinant ATP1A1 Antibody (Ab128631)
Recombinant ATP1A1 Antibody (Ab128631) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant ATP1A1 Antibody (Ab128631) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant ATP1A1 Antibody (Ab128631) (1/200 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.
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IP: Recombinant ATP1A1 Antibody (Ab128631)
Recombinant ATP1A1 Antibody (Ab128631) - IP
NaK ATPase was immunoprecipitated from 0.4 mg of TT whole cell lysate with Recombinant ATP1A1 Antibody (Ab128631) at 1/50 dilution
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant ATP1A1 Antibody (Ab128631) IP in TT whole cell lysate
Lane 2: PBS instead of Recombinant ATP1A1 Antibody (Ab128631) in TT whole cell lysate
Lane 3: TT whole cell lysate, 10 μg (input)
Exposure: 120 s.
查看阿拉丁官網此產品相關對應頁面：https://www.aladdin-e.com/zh_cn/Ab128631.html
關鍵字： 鈉鉀ATP酶蛋白a1(內參) 重組單克隆抗體 ; 鈉鉀ATP酶蛋白a1重組單克隆抗體 ; 鈉鉀ATP酶蛋白a1抗體 ; 重組ATP1A1抗體
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