中文名：Synthetic Blocking Buffer, ELISA
英文名：Synthetic Blocking Buffer, ELISA
純度：Ready-to-use (RTU)
貨號：S494401
存儲溫度：2-8°C儲存,避光
運輸條件：低溫運輸
產(chǎn)品介紹：
Product Characteristics?
The Synthetic Blocking Buffer, ELISA is a ready-to-use solution for blocking of the remaining free binding sites after microplate coating in ELISAs. The buffer blocks all potential binding sites of nonspecific interaction and reduces the background signal, improving the signal-to-noise ratio.?
The Synthetic Blocking Buffer, ELISA reduces the risk of false positive reactions in the assay, while avoiding the current safety hazards related to the use of bovine biological material, for instance BSA.
Composition & Properties?
The Synthetic Blocking Buffer is a ready-to-use buffer. The buffer contains no proteins, and crossreactivity is therefore avoided.
Working Procedure?
1.Coat the microtiter plate according to the usual procedure. It is recommended to dilute the biomolecule in a fresh carbonate buffer, pH 9.6.?
2.After plate coating, rinse the microwells with 3 x 300 μl PBS buffer.?
3.Dispense the Synthetic Blocking Buffer into each well making sure wells are completely full.?
4.Incubate for 5-10 minutes at room temperature.?
5.Rinse with 3 x 300 μl washing buffer optimized for the assay.?
6.Continue the plate processing using the usual procedure.
Tips & Tricks?
? In case of a demand for stabilization of coated ELISA plates, our one step blocker & stabilizer, WellChampion (cat. no. W494467) is recommended.?
? For membranes, the Synthetic Blocking Buffer, Blotting (cat. no. S494457) is recommended.?
Handling & Storage?
? Store solution at 2-8 °C.
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關(guān)鍵字： 合成封閉緩沖液，ELISA
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