PNGase F產(chǎn)品信息
別稱：PNGase F,PNGase F,PNGF
物種：Elizabethkingia miricola
屬性：Protein
標(biāo)記：Unconjugated
產(chǎn)品描述（Product Details）
PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F is an amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides.
應(yīng)用說明（Application）
Leaves N-glycan core oligosaccharides intact and suitable for further analysis.
Non-recombinant with no detectable endoglycosidase F1, F2 or F3 contamination.
單位定義（Unit Definition）
One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 10 μg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 20 μl.
純度（Purity）
>90% as determined by SDS-PAGE.
酶活（Enzyme Activity）
>500 U/μL
內(nèi)毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
注意事項（Notes）
To deglycosylate a native glycoprotein, longer incubation time as well as more enzyme may be required.
PNGase F will not cleave N-linked glycans containing core α1-3 Fucose.
制劑（Formulation）
Supplied as 0.2 μm filtered solution in 20 Mm Tris, 50 mM NaCl, 5 mM EDTA, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
運輸（Shipping）
This product is supplied and shipped with blue ice, please inquire the shipping cost.
存儲（Storage）
This product is stable after storage at:
The product MUST be stored at -20°C or lower upon receipt.
-20°C for 12 months under sterile conditions.
PNGase F分子背景
PNGase F（肽 N- 糖苷酶 F）來源于 Elizabethkingia miricola，是一種高效的酰胺水解酶，可以裂解由天冬酰胺接的高甘露糖、雜合和復(fù)雜的寡糖糖蛋白。PNGase F 的切割位點為糖蛋白內(nèi)側(cè) N- 乙酰葡萄糖胺（GlcNAc）和天冬酰氨殘基之間的酰胺鍵，同時將酶解后蛋白上的天冬氨酰轉(zhuǎn)化為天冬氨酸。
關(guān)鍵字： PNGase F;PNGaseF酶;PNGase F蛋白;ACRO;百普賽斯;
百普賽斯集團ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國生物科技公司，是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領(lǐng)域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團業(yè)務(wù)遍布全球，橫跨亞洲、北美洲、歐洲，在中國、美國、瑞士等12個城市設(shè)有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計服務(wù)客戶超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關(guān)系。集團旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學(xué)等。