Usages:
For detection of molds, yeasts and total bacteria , commonly used in aseptic filling empty PET bottle production line of sterility testing.
Principle: peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; glucose carbon source; potassium dihydrogen phosphate as a buffer; provide essential trace elements of magnesium sulfate; ammonium sulfate osmotic equilibrium can be maintained and conducive to the growth target bacteria
Formulation (per liter):
Glucose :20.0g
Ammonium sulfate:2.0g
Magnesium sulfate:1.0g
Peptone:2.0g
Yeast extract powder: 3.5g
Sodium dihydrogen phosphate: 1.0g
Final pH 6.5 ± 0.2
How to use:
1. Weight 29.5g of product, adding 1L of distilled water or deionized water,stirring heated to boiling until complete dissolution, dispensing tube or flask, 121 ℃ sterilization for 15minutes.
2. Inoculated the treated sample to LG medium, according to the specific requirements of sterility testing, vaccination appropriate sample size, the number of pieces of medium and positive control, the medium was placed in 28 ℃ ~ 32 ℃ prescribed time training 13 ~ 15d.
3. Observe the results.
Quality Control:
Appearance:
Powder: light yellow powder
Prepared broth: yellow, clear and transparent without precipitation.
Biology testing: The following strains were inoculated after 28 ℃ ~ 32 ℃ cultured 13 ~ 15d growth in the following table:
Quality control
Bacteria Name Bacteria No. growth characteristics
Pseudomonas aeruginosa ATCC27853 turbid broth
Staphylococcus aureus ATCC6538 turbid broth
Bacillus cereus ATCC63303 turbid broth
Klebsiella pneumonia ATCC46117 turbid broth
Hyphae of Aspergillus niger ATCC16404 flocculent, liquid black spores can
Candida albicans ATCC10231 turbid broth
Specifications: 10kg / bag.
Storage: Store in a dark, cool and dry place,sealed the product immediately after use.
Storage period of three years