CD 293 TGE Medium, 1X, Liquid
產(chǎn)品描述（Description）
CD 293 TGE培養(yǎng)基是一種有專利的、化學(xué)定義的、無動(dòng)物來源的培養(yǎng)基，專門用于293細(xì)胞的高密度懸浮培養(yǎng)和轉(zhuǎn)染。質(zhì)粒DNA在適應(yīng)于該培養(yǎng)基的HEK293細(xì)胞中的瞬時(shí)表達(dá)可以獲得高體積產(chǎn)率的靶蛋白。建議將293 Expression MAX-1和Feed X Supplement與BPM BPfectin一起使用，以獲得更高的蛋白質(zhì)表達(dá)率。
CD 293 TGE培養(yǎng)基專為以下用途配制：
1. 懸浮液HEK293（293T、293EBNA、293F）瞬時(shí)表達(dá)
2. HEK 293穩(wěn)定蛋白表達(dá)細(xì)胞系的懸浮培養(yǎng)
3. HEK293細(xì)胞在生物反應(yīng)器中的大規(guī)模高密度生長
產(chǎn)品特性（Product Features）
1. Support suspension HEK 293(293T, 293EBNA, 293F) transient expression
2. >200% cell growth and expression performance compared competitive alternatives
3. Prepared ready-to-use, with no supplementation required
4. Protein free, animal component free, chemically define and regulatory friendly
5. Extensive support by cell culture expert
產(chǎn)品參數(shù)（General Specification）
操作流程（Usage Protocol）
① Before transfection, passage 293 cells with CD 293 TGE Medium for at least 3 round from an adaptation procedure, seeding density greater than 0.5 ×106 cells/ml, cell viability >95%. Culture at 150-180 rpm depending on your orbital shaker design and single cell distribution status.
② Seed 293 cells at 0.5 ×106 cells/ml and transfer to pre-warmed fresh medium, subsequently grow the cells to 1.5 to 2.2 ×106 cells/ml.
③ The day before transfection, dilute the culture with fresh medium to 0.8 to 1.0 ×106 cells/ml and cell density at transfection should range from 1.5 × 106 to 2.0 × 106 cells/ml, provided the doubling time is 24 h and viability is greater than 95%.
④ On the day of transfection, using sterile 150 mM NaCl solution to dilute DNA plasmid and add 293 Expression MAX-1 (Catalog # EXP-711) according to the instruction.
⑤ Add BPfectin (Catalog # TF-1157) at ratio of 3:1 (BPfectin : DNA; volume/weight) to DNA solution mix well with vortex for 3 sec.Note: DNA dosage for transfection is 1 ug per 1 million cells in culture, and volume of DNA-BPfectin complexes is 5%(v/v) of culture. (e.g. 500 μL to 10 mL culture).
⑥ Incubate the complexes at RT for 10 min before transfection.
⑦ Add DNA-BPfectin complexes to cells at 5% volume ratio mix well gently.
⑧ 24 hours post transfection, add Feed X Supplement (Catalog # CF-1116) to culture at 10% volume ratio (e.g. 1 mL to 10mL culture).
⑨ Harvest for purification typically 7 days post transfection or when cell viability drops below 55%.
ACRO培養(yǎng)基用戶評(píng)價(jià)
關(guān)鍵字： 培養(yǎng)基;細(xì)胞培養(yǎng)基;TGE瓊脂培養(yǎng)基;ACRO;百普賽斯;
百普賽斯集團(tuán)ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國生物科技公司，是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領(lǐng)域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺(tái)型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團(tuán)業(yè)務(wù)遍布全球，橫跨亞洲、北美洲、歐洲，在中國、美國、瑞士等12個(gè)城市設(shè)有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計(jì)服務(wù)客戶超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關(guān)系。集團(tuán)旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學(xué)等。