Human HGF ELISA Kit

背景（Background）

ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human HGF that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.

A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.

ELISA Kits are manufactured in a GMP-like facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.

原理（Assay Principles）

This assay kit is used to measure the concentrations of human HGF by employing a standard sandwich-ELISA format. The microplate in the kit has been pre-coated with Anti- HGF Antibody. First add the standard samples provided in kit and your samples to the plate, next add detection antibody Biotin-Anti-HGF Antibody to the plate, incubate and wash the wells. After wash add HRP-Streptavidin to the plate, incubate and wash the wells. Lastly load the substrate into the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm and 630nm.

應(yīng)用說明（Application）

The kit is developed for quantitative detection of human HGF in serum, plasma and cell culture supernates.

It is for research use only.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存儲(chǔ)（Storage）

Unopened kit should be stored at 2℃-8℃ upon receiving. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

背景（Background）：HGF

Hepatocyte growth factor (HGF) is a paracrine cellular growth, motility and morphogenic factor. It is secreted by mesenchymal cells and targets and acts primarily upon epithelial cells and endothelial cells, but also acts on haemopoietic progenitor cells and T cells. It has been shown to have a major role in embryonic organ development, specifically in myogenesis, in adult organ regeneration, and in wound healing.