Human TNF-alpha Protein, His Tag, active trimer (MALS verified)

分子別名（Synonym）

DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human TNF-alpha Protein, His Tag (TNA-H5228) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # NP_000585.2).

Predicted N-terminus: Val 77

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 18.3 kDa. The protein migrates as 17-19 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

腫瘤壞死因子α（TNFα）是一種主要由單核細(xì)胞和巨噬細(xì)胞產(chǎn)生的細(xì)胞因子。它存在于滑膜細(xì)胞和組織中的巨噬細(xì)胞中。TNFα的主要作用是調(diào)節(jié)免疫細(xì)胞。TNFα能夠誘導(dǎo)細(xì)胞凋亡、誘導(dǎo)炎癥、抑制腫瘤發(fā)生和病毒復(fù)制。TNFα產(chǎn)生的失調(diào)與多種人類疾病有關(guān)，包括嚴(yán)重抑郁癥、阿爾茨海默病和癌癥。重組TNFα作為免疫刺激劑在INN tasonermin下使用。TNFα可在惡性腫瘤中異位產(chǎn)生，在引起繼發(fā)性高鈣血癥和與過量產(chǎn)生相關(guān)的癌癥中與甲狀旁腺激素相似。