Human Mucin-1 / MUC-1 (1036-1155) Protein, His Tag

分子別名（Synonym）

Mucin-1,MUC-1,CD227

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human Mucin-1 (1036-1155), His Tag (MU1-H52H5) is expressed from human 293 cells (HEK293). It contains AA Leu 1036 - Gly 1155 (Accession # P15941-1).

Predicted N-terminus: Leu 1036 (partial α chain) & Ser 1098 (partial β chain)

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus. The mature form of Mucin-1 is a non-covalent heterodimeric complex with the proteolytically cleaved partial α and partial β chain. Each partial α and partial β chain has a calculated MW of 7.2 kDa (partial α chain) and 8.2 kDa (partial β chain). The protein migrates as 13-15 kDa (partial α chain) and 10 kDa (partial β chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

Mucin-1蛋白在上皮細(xì)胞中具有多種功能，包括細(xì)胞保護(hù)、轉(zhuǎn)移過(guò)程中的外滲、管腔結(jié)構(gòu)的維持和信號(hào)轉(zhuǎn)導(dǎo)。MUC17含有一個(gè)延伸的、重復(fù)的細(xì)胞外糖基化結(jié)構(gòu)域和一個(gè)羧基末端，具有兩個(gè)EGF樣結(jié)構(gòu)域，一個(gè)SEA模塊結(jié)構(gòu)域、一個(gè)跨膜結(jié)構(gòu)域和具有潛在絲氨酸和酪氨酸磷酸化位點(diǎn)的細(xì)胞質(zhì)結(jié)構(gòu)域。通過(guò)其C末端與PDZK1相互作用，這種相互作用對(duì)正確定位很重要?？赡茉诰S持粘膜表面的穩(wěn)態(tài)方面發(fā)揮作用。