Human TFPI Protein, His Tag (active enzyme)

分子別名（Synonym）

TFPI,LACI,TFPI1,EPI,TFI

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human TFPI, His Tag (TFI-H5226) is expressed from human 293 cells (HEK293). It contains AA Asp 29 - Lys 282 (Accession # NP_006278.1).

Predicted N-terminus: Asp 29

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 30.0 kDa. The protein migrates as 41-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

組織因子途徑抑制物（TFPI）也稱為外源途徑抑制物（EPI）、脂蛋白相關(guān)凝血抑制物（LACI），是一種由血管內(nèi)皮細(xì)胞合成的血漿蛋白酶抑制物，其中一部分與這些細(xì)胞的糖胺聚糖有關(guān)。TFPI是一種單鏈多肽，可以可逆地抑制因子Xa（Xa）和凝血酶（因子IIa）。TFPI是一種分泌蛋白，具有N端酸性區(qū)、由兩個接頭區(qū)分隔的三個Kunitz（K）結(jié)構(gòu)域和一個C端堿性區(qū)。第一個K結(jié)構(gòu)域抑制與組織因子（TF）復(fù)合的凝血因子VIIa；第二個K結(jié)構(gòu)域抑制因子Xa；第三個K結(jié)構(gòu)域與肝素結(jié)合；C終端基本區(qū)域可以具有多種功能。例如，它在TFPI與細(xì)胞表面的結(jié)合中起著重要作用。