| 名稱 | Protocatechuic acid |
| 描述 | Protocatechuic acid (3, 4-Dihydroxybenzoic acid) (3, 4-dihydroxybenzoic acid) is a natural phenolic compound found in many edible and medicinal plants. Recent studies indicate that it could be used as a protective agent against cardiovascular diseases and neoplasms. The mechanism of its action is mostly associated with antioxidant activity, including inhibition of generation as well as scavenging of free radicals and up-regulating enzymes which participate in their neutralization. |
| 細胞實驗 | Protocatechuic acid is dissolved in DMSO. Dilutions of Protocatechuic acid (2, 5, 10, 20, 50, and 100 μM) are prepared from stock solutions, with serum-free culture medium. Equal volumes of each solution are mixed with Aβ1-42 (10 μM), then incubated for 24 h on a thermoblock, with continuous agitation, and then exposed to PC12 cells for 24 h to test whether Protocatechuic acid can prevent cell death triggered by Aβ. Cell viability is determined by MTT reduction assay. Cells are treated with 200 μL per well of MTT solution (final concentration, 0.5 mg/mL in DMEM-Glutamax medium) for 3 h, at 37°C, with 5% CO2. The dark blue formazan crystals that formed are solubilized with 100 μL per well of DMSO, for 30 min. Absorbance is measured at 540 nm, with a microplate reader. Results are expressed as the percentage of MTT reduction in relation to the absorbance of control cells at 100%. |
| 激酶實驗 | AChE activity investigation is carried out in a reaction mixture containing 50 μL of tissue homogenate, 50 μL of 5, 5'-dithiobis-(2-nitrobenzoic) acid (DTNB), 1175 μL of 0.1 M phosphate-buffered solution, pH 8.0. After incubation for 20 min at 25°C, 25 μL of acetylthiocholine iodide solution is added as the substrate. The AChE activity is determined as changes in absorbance reading at 412 nm for 3 min at 25°C and using a UV/Visible spectrophotometer. |
| 體外活性 | Protocatechuic acid 抑制Aβ和αS的聚集，破壞其已形成的纖維結(jié)構(gòu)，預(yù)防由Aβ和αS誘導(dǎo)的毒性引起的PC12細胞死亡[3]。 |
| 體內(nèi)活性 | Protocatechuic acid 能在不改變小鼠運動活動的情況下，防止應(yīng)激誘導(dǎo)的強迫游泳試驗中的靜止時間。此外，Protocatechuic acid 還能減輕 ARS 小鼠血清皮質(zhì)酮的升高和脂質(zhì)過氧化反應(yīng)，并恢復(fù)大腦皮層和海馬中的酶抗氧化劑[1]。給大鼠注射鎘并用前列腺素和Protocatechuic acid （10-20 mg/kg）治療后，其 BChE 活性明顯降低。鎘和前列腺素或Protocatechuic acid （10-20 mg/kg）處理大鼠后，MDA 水平明顯下降[2]。 |
| 存儲條件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Shipping with blue ice/Shipping at ambient temperature. |
| 溶解度 | H2O : 5 mg/mL (32.44 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (12.98 mM), Sonication is recommended.
DMSO : 96 mg/mL (622.89 mM), Sonication is recommended.
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| 關(guān)鍵字 | Protocatechuic acid | Inhibitor | inhibit | free radical | Endogenous Metabolite |
| 相關(guān)產(chǎn)品 | Formamide | Guanidine hydrochloride | Glycerol | Aceglutamide | Malic acid | DL-Lysine | Sucrose | Nicotinamide riboside malate | Gluconate Calcium | Thymidine | Corn starch | D(+)-Raffinose pentahydrate |
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