Mouse IL-4 Protein, His Tag

分子別名（Synonym）

IL4,BCGF1,BSF1

表達區(qū)間及表達系統(tǒng)（Source）

Mouse IL-4, His Tag (IL4-M52H5) is expressed from human 293 cells (HEK293). It contains AA His 21 - Ser 140 (Accession # P07750-1).

Predicted N-terminus: His 21

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 15.4 kDa. The protein migrates as 17-20 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

白細胞介素-4是一種誘導(dǎo)幼稚輔助T細胞（Th0細胞向Th2細胞）分化的細胞因子。在IL-4和IL-13的存在下，Th1型反應(yīng)中產(chǎn)生的細胞因子，特別是在過敏和寄生蟲感染期間，巨噬細胞會被不同程度地激活，這種細胞因子是白細胞介素4受體的配體。白細胞介素4受體也與IL13結(jié)合，這可能導(dǎo)致該細胞因子和IL13的許多重疊功能。STAT6是一種信號轉(zhuǎn)導(dǎo)子和轉(zhuǎn)錄激活子，已被證明在介導(dǎo)這種細胞因子的免疫調(diào)節(jié)信號中起著核心作用。最近，研究人員發(fā)現(xiàn)，細胞因子IL-4在幾種基因缺陷小鼠品系（包括Itk、KLF2、CBP和Id3）胸腺中先天CD8+T細胞的發(fā)育中起著關(guān)鍵作用，這些小鼠品系以前沒有接觸過抗原。