Mouse IL-3 Protein, His Tag (MALS & SPR verified)

分子別名（Synonym）

IL3,MCGF,MGC79398,MGC79399,MULTI-CSF,Interleukin-3

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Mouse IL-3 Protein, His Tag (IL3-M52H8) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Cys 166 (Accession # P01586-1).

Predicted N-terminus: Ala 27

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 17.6 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

白細(xì)胞介素3也稱(chēng)為IL-3，是一種在人類(lèi)中由IL3基因編碼的蛋白質(zhì)。白介素-3（IL-3）是一種白介素，是一種生物信號(hào)（細(xì)胞因子），作為免疫系統(tǒng)的一部分，可以改善身體對(duì)疾病的自然反應(yīng)。它通過(guò)與白細(xì)胞介素-3受體結(jié)合起作用。IL-3受體是IL-3特異性α鏈和常見(jiàn)β鏈βc的異二聚體，βc也被GM-CSF和IL-5使用。[3] IL-3的結(jié)合也可能涉及用似乎對(duì)IL-3具有特異性的βIL-3鏈取代βc。IL-3與其同源受體的結(jié)合誘導(dǎo)Jak2的激活、多種Stats的磷酸化和PI3K/Akt通路。IL-3可能在哮喘相關(guān)氣道炎癥的發(fā)展中起重要作用。人IL-3基因編碼一種152個(gè)氨基酸長(zhǎng)的蛋白質(zhì)，天然存在的IL-3是糖基化的。人類(lèi)IL-3基因位于5號(hào)染色體上，距離GM-CSF基因僅9千堿基，其功能與GM-CSF非常相似。