Mouse Siglec-10 Protein, His Tag

分子別名（Synonym）

SIGLEC10,MGC126774,PRO940,Siglec10,SLG2

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Mouse Siglec-10, His Tag (SI0-M52H7) is expressed from human 293 cells (HEK293). It contains AA Met 18 - Lys 543 (Accession # Q80ZE3-1).

Predicted N-terminus: Met 18

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 60.7 kDa. The protein migrates as 40 kDa,50 kDa and 75-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in 25 mM MES, 150 mM NaCl, pH5.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

Siglecs（唾液酸結(jié)合Ig樣凝集素）是具有粘附分子樣結(jié)構(gòu)的Ig超家族的一個(gè)獨(dú)特子集。我們在這里描述了siglec蛋白家族的一個(gè)新成員，它具有相似的結(jié)構(gòu)，包括五個(gè)Ig樣結(jié)構(gòu)域、一個(gè)跨膜結(jié)構(gòu)域和一個(gè)含有兩個(gè)ITIM信號(hào)基序的細(xì)胞質(zhì)尾部。Siglec-10是通過哮喘嗜酸性粒細(xì)胞EST庫的數(shù)據(jù)庫挖掘鑒定的。Siglec-10-VAP-1相互作用似乎介導(dǎo)淋巴細(xì)胞與內(nèi)皮細(xì)胞的粘附，并有可能通過酶終產(chǎn)物改變炎癥微環(huán)境。