溶于水、乙醇。溶液為橙黃色帶綠色熒光。pH值為8.4～10.4則無熒光。能鑲嵌于兩個(gè)相鄰的堿基對(duì)之間，使部分雙螺旋DNA松開，該兩個(gè)堿基對(duì)的距離拉大一倍。這樣的DNA復(fù)制過程中，會(huì)使DNA鏈增加或缺失一個(gè)堿基，造成移碼突變。Colour Index Number 46005 用作熒光指示劑，腫瘤細(xì)胞及細(xì)菌、核酸染色劑及移碼突變的誘變劑。 吖啶橙是一種熒光色素，吖啶橙激發(fā)濾光片波長488nm。阻斷濾光片波長515nm。吖啶橙與細(xì)胞中DNA和RNA結(jié)合量存在差別，可發(fā)出不同顏色的熒光（即著色特異性），這是由于DNA是個(gè)高度聚合物，吸收熒光物質(zhì)的位置較少，發(fā)綠色熒光，而RNA聚合度低，能和熒光物質(zhì)結(jié)合的位置多，故發(fā)紅色熒光。
用途 適合 檢測凝膠電泳分離的核酸 熒光和落射熒光顯微鏡 流式細(xì)胞術(shù)分析線粒體和溶酶體 凋亡研究中的DNA染色 吖啶橙是一種細(xì)胞可滲透的異染色熒光陽離子染料，可嵌入 DNA 和 RNA，用于熒光和表觀顯微鏡檢測。吖啶橙染料已被用于通過流式細(xì)胞術(shù)分析線粒體和溶酶體含量，表征多藥耐藥性以及檢測大鼠胸腺細(xì)胞凋亡過程中線粒體質(zhì)量的變化。 Acridine Orange hemi (zinc chloride) salt is a cell-permeable metachromatic fluorescent dye that stains DNA and RNA. This dye is also an inhibitor of RNA polymerase.
Fluorescent dye that stains DNA and RNA
Application Suitable for detection of nucleic acids separated by gel electrophoresis fluorescence and epifluorescence microscopy analysis of mitochondria and lysosomes by flow cytometry DNA staining in apoptosis studies Acridine Orange, a cell-permeable metachromatic fluorescent cationic dye that intercalates DNA and RNA, is used in fluorescence and epiflouresence microscopy. Acridine Orange dye has been used to analyze mitochondria and lysosomal content by flow cytometry, characterize multidrug resistance, and measure changes in mitochondrial mass during apoptosis in rat thymocytes. |