Human / Mouse FGF-8b Protein, His Tag

分子別名（Synonym）

FGF8,Fibroblast growth factor 8,FGF-8，Androgen-induced growth factor，Heparin-binding growth factor 8 ,AIGF

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human / Mouse FGF-8b Protein, His Tag (FGB-H52H1) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Arg 215 (Accession # P55075-3).

Predicted N-terminus: Gln 23

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 24.3 kDa. The protein migrates as 35-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

無菌（Sterility）

Negative

支原體（Mycoplasma）

Negative.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 24 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

FGF8被鑒定為一種雄激素誘導(dǎo)的生長因子，由乳腺癌細(xì)胞系分泌，在胚胎發(fā)育過程中廣泛表達(dá)。它已被證明介導(dǎo)胚胎上皮-間充質(zhì)轉(zhuǎn)化，并在原腸胚形成和中腦/后腦、咽、心臟、泌尿生殖和肢體結(jié)構(gòu)的早期組織和分化中起著關(guān)鍵作用。在成年期，FGF8的表達(dá)受到更多限制，但在激素性癌癥中，它經(jīng)常被激活。FGF8亞家族，包括FGF8、FGF17和FGF18，不僅在前列腺、乳腺癌癥中檢測到，而且在肝細(xì)胞癌（HCC）中也檢測到。FGF8、FGF17和FGF18參與HCC中的自分泌和旁分泌信號傳導(dǎo)，并增強腫瘤細(xì)胞在應(yīng)激條件下的存活率、惡性行為和新生血管生成。脊椎動物FGF8基因通過選擇性剪接產(chǎn)生多種蛋白質(zhì)亞型。兩種進(jìn)化上保守的剪接形式FGF8a和FGF8b表現(xiàn)出不同的生物活性，其中FGF8b由于對FGF受體具有更高的親和力而具有更強的誘導(dǎo)活性。