不溶于冷水，微溶于熱水呈艷紅光藍色，微溶于乙醇呈艷藍色。遇濃硫酸呈橙紅色。其水溶液加氫氧化鈉呈紫色。 用于檢測電泳后的蛋白條帶。 比萘酚藍黑B（Naphthol Blue Black B）和溴酚（Bromophenol）靈敏5-10倍（ 能檢測到膠基質(zhì)上0.5 μg/cm2 蛋白質(zhì))。

application：

Brilliant Blue R is used for detection of protein bands following electrophoresis. It is a sensitive stain for protein detection in polyacrylamide gels, typically giving blue bands on a clear background with a sensitivity of 50-100 ng/band. It may be combined with other stains, such as silver stain, to distinguish different types of proteins. It displays metachromasia by appearing pink-red, rather than blue, when binding certain proteins, such as collagen and histone H1.